摘要
目的建立测定六经头痛片中胡薄荷酮的高效液相色谱法。方法采用Agilent Zorbax SB C18色谱柱（250 mm×4.6mm,5μm）;以乙腈–0.05%磷酸水溶液为流动相,进行梯度洗脱;检测波长256 nm;柱温30℃;体积流量1.0 mL/min;进样量10μL。结果胡薄荷酮在0.442 3-1 105.8 ng与峰面积线性良好（r=0.999 9）,平均回收率为97.59%,RSD值为1.54%（n=6）。结论建立的方法专属性强、准确、快速,可有效地控制六经头痛片的质量。
Objective To establish an HPLC method for the determination of pulegone in Liujing Toutong Tablets. Methods The separation of pulegone was carried out by using Agilent Zorbax SB C18 column （250 mm x 4.6 mm, 5μm） with the mobile phase containing acetonitrile - 0.05% phosphoric acid aqueous with gradient elution. The detective wavelength was set at 256 nm. The flow rate was 1.0 μL/min with injection volume of 10 μL. Results The linearity range ofpulegone was 0.442 3 - 1 105.8 ng （r=0.999 9）, and the average recovery was 97.59% with RSD value of 1.54% （n=6）. Conclusion The method is simple and accurate with good recovery and repeatability, and suitable for the quality control of Liujing Toutong Tablets.
出处
《现代药物与临床》
CAS
2014年第5期500-502,共3页
Drugs & Clinic
