摘要
以国内外的10个双孢菇菌株为试材，利用ISSR分子标记技术对其进行亲缘关系分析。结果表明：从117个ISSR引物中筛选出11个条带清晰重复性好的引物，对10个双孢菇菌株的基因组DNA进行扩增，共获得73条清晰条带，其中多态性条带51条，多态性位点百分率为69．86％。利用NTSYSpc2．10软件计算菌株间Nei遗传距离为0．5479～0．9589，以平均连锁法（UPGMA）进行聚类分析，可将10个双孢菇菌株分为4个类群：类群Ⅰ为国内的2个菌株258和2796；类群Ⅱ是引自荷兰的HRLM；类群Ⅲ包括引自美国的L901、5113、XXX和2200；类群Ⅳ包括引自荷兰的H901、F3F和HA15。引自美国和荷兰的双孢菇菌株之间亲缘关系较近，相似性达0．85以上，而与我国的双孢菇亲缘相似性仅为0．61。因此，国外优质双孢菇菌株的引进对于增加我国双孢菇遗传多样性具有重要作用。
The genetic relationship of 10 Agaricus bisporus cultivation strains was analyzed by inter-simple sequence repeats （ISSR）. The results showed that 11 effective primers were screened from 117 ISSR primers. A total number of 73 clear bands were amplified from the 10 Agaricus bisporus strains, among which 51 bands were polymorphic and the polymorphic loci ratio was 69.86%. The genetic similarity coefficients among 10 strains were between 0. 5479 and 0. 9589 using NTSYSpc 2. 10. The clustering analysis conducted with UPGMA indicated that 10 Agaricus bisporus strains could be divided into four groups： the first group included two domestic strains i. e. 258 and 2796; the second group only included HRLM from Netherlands;the third group was consisted of L901,5113, XXX and 2200 from American;the fourth group was consisted of H901, F3F and HA15 from Netherlands. In contrast to two domestic strains, the genetic relationship of Agaricus bisporus strains between from American and from Netherlands was closer and the genetic similarity coefficients was higher than 0. 85, but the genetic similarity of Agaricus bisporus from China was only 0. 61. The introduction of foreign high-quality Agaricus bisporus strains would play an important role to increase Agaricus bisporus genetic diversity in our country.
出处
《北方园艺》
CAS
北大核心
2014年第11期96-99,共4页
Northern Horticulture
基金
国家"948"计划资助项目(2012-Z19)
山东省农业良种工程重大课题资助项目
山东省现代农业产业技术体系食用菌产业创新团队资助项目(SDAIT-11-011-15)
关键词
双孢菇
ISSR
亲缘关系
聚类分析
Agaricus bisporus
ISSR
genetic relationship
cluster analysis
