摘要
目的研究降酚菌的降解影响因素,诱变选育出高效苯酚降解菌,并确定此株高效菌的降解特性.方法从3种土样中分离纯化出一株能降解较高质量浓度苯酚的野生菌P4,通过菌落形态及显微镜观察作初步鉴定.采用紫外诱变的方法,从诱变后的正突变菌株中选取一株降解率最高且生长旺盛的菌株,命名为高效苯酚降解菌P4S,利用正交试验法测试其降解特性.结果初步鉴定野生菌株P4为酵母菌,该菌能在48 h内将800 mg/L的苯酚完全降解.经紫外诱变选育出的高效降酚菌P4S,能降解1 600 mg/L的苯酚,并可耐受1 700 mg/L的苯酚.由正交试验与验证性试验结果相比较,得出其在苯酚废水中的最佳降解条件为150 r/min,36℃,pH为6,生物投加量为5%,降解率可达85%.结论经诱变选育出的高效苯酚降解菌能降解更高质量浓度的苯酚,环境适应范围更广,更适用于实际生产.
This paper discussed the influencing factor of the degradation efficiency of phenol strain and breeding the degradation efficiency of phenol strain through the ultra-violet mutation method. In this research we separated and purified a wild strain named P4 which can degrade higher levels of phenol, preliminary identification on the wild strain through observing morphology and cell morphous under microscope. Screening an eugonic strain with highest degradation rate from the positive mutant strain, named P4S. We chose the factors which is more influenced, carry out orthogonal test to find the biodegradation characteristics of P4S. After the preliminary appraisal ,P4 is microzyme. It can fully biodegrade 800 mg/L phenol in 48 h. UV-induced mutation strain P4S can degrade 1600 mg/L phenol and tolerate 1 700 mg/L phenol. Orthogonal test results show that the best conditions for degrading phenolic waste water are 150 r/rain ,36 ℃ ,pH6, 5 % dosage. On this condition, the phenol degradation rate can achieve 85 %. The UV mutagenesis higher efficiency of phenol degradation strain can degradate the higher phenol rate and adapt a wider range of envi- ronmental condition which is more applicable to industry processing.
出处
《沈阳建筑大学学报（自然科学版）》
CAS
北大核心
2014年第3期547-552,共6页
Journal of Shenyang Jianzhu University：Natural Science
基金
国家自然科学基金项目(41072190)
关键词
自然土壤
降酚菌
紫外诱变
含酚废水
natural soil
phenol-degradation strain
UV-induced mutation
phenolic wastewater
