摘要
通过三水平三因素的完全组合方法,对分离到的苯酚降解菌Klebsiella sp.C1、Bacillus sp.F6和Arthrobacter sp.E2进行高效苯酚降解菌群的构建和评价。最佳菌群JF的最佳体积组合比例为V（Klebsiella sp.C1）：V（Bacillus sp.F6）：V（Arthrobacter sp.E2）=2：1：3。与单菌株相比,菌群JF具有更强的环境适应能力,能够适应15-45℃,pH 4-10,NaCl浓度≤60 g·L-1的生长环境,最大耐受苯酚浓度可达2500 mg·L-1,能够保持长时间及较高的苯酚降解效率,且能够多周期高效运行。菌群JF降解苯酚的整个反应过程中,培养液酸碱度的变化平缓,表明其具有较好的内稳性并在处理复杂含酚废水方面具有良好的应用前景。
Bacterial consortiums with isolated bacteria Klebsiella sp. C 1, Bacillus sp. F6, and Arthrobacter sp.E2 were constructed and evaluated for phenol degradation with an orthogonal experimental design. Thecombination was optimized by mixing a volume proportion of V（Klebsiellasp. CI）： V（Bacillus sp. F6）： V（Arthrobacter sp. E2）=2：1：3Compared with any single bacterium, the bacterial consortium shows better activity under growing condition of15℃ to 45℃, pH 4-10, NaC1 concentration ≤60 g.L-1 and phenol concentration ≤2500 g.L-1, which canretain high phenol degradation activity for a long time. Results show that pH change during the treatmentprocess is insignificant to the biodegradation performance, which suggests that the bacterial consortium is stablein the system and may have a potential in phenol bioremediation in contaminated environment.
出处
《高校化学工程学报》
EI
CAS
CSCD
北大核心
2014年第2期298-304,共7页
Journal of Chemical Engineering of Chinese Universities
基金
广西自然科学基金项目(2013GXNSFAA253002)
广西高等学校科研资助项目(200I03YB004)
广西大学基金项目(XB2100099)
广西环境工程与保护评价重点实验室开放课题(桂科能0804K018)
广西研究生教育创新计划资助项目(105931003024)
关键词
苯酚
生物降解
复合菌群
phenol
biodegradation
bacterial consortium
