摘要
探讨中药苦碟子注射液对缺血性脑卒中火毒证大鼠脑组织蛋白质表达的调控作用。采用角叉菜胶复合大脑中动脉阻塞(MCAO)方法制备缺血性脑卒中火毒证大鼠模型,按随机数字表法将SD大鼠分为药物组、模型组和假手术组。采用TTC染色法和HE染色法对脑组织进行大体观察和病理形态学观察;利用双向凝胶电泳技术寻找缺血性脑卒中火毒证大鼠和假手术大鼠之间与疾病相关的差异表达蛋白,并观察应用苦碟子注射液连续干预72 h后,对差异蛋白表达水平的影响。结果显示,缺血性脑卒中火毒证大鼠与假手术大鼠间有差异表达蛋白,连续给予具有清热解毒通络作用的苦碟子注射液能够减轻脑组织缺血形态学改变,调节部分差异蛋白表达水平。
This study is to investigate the modulation of Kudiezi（KDZ） injection on differential protein expression in cerebral cortex of rats with cerebral ischemic stroke and heat toxin syndrome established by intraperitoneal injection of carrageenan and middle cerebral artery occlusion（MCAO） methods.According to random number table rats were divided into three groups： drug group,model group and sham group.The tripheye tetrazolium chloride（TTC） staining and HE staining were used to observe brain tissue injury of rats.After therapeutic intervention with above drug for seventy-two hours,the level of differential protein expression was analyzed by two-dimensional gel electrophoresis（2-DE）.The results show that there are differential protein expressions between cerebral ischemic stroke and heat toxin syndrome rats and sham rats.Furthermore,as a Chinese medicine injection with effect of clearing heat,resolving toxin and dredging collaterals,KDZ injection can decrease alleviate morphological changes of cerebral ischemia,regulate the levels of some differential proteins expression.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2014年第10期1874-1879,共6页
China Journal of Chinese Materia Medica
基金
国家重点基础研究发展计划(973)项目(2012CB518406)
北京中医药大学创新团队发展计划(2011-CXTD-23)
关键词
苦碟子注射液
缺血性脑卒中
火毒证
电泳
蛋白质组
Kudiez injection
cerebral ischemic stroke
heat toxin syndrome
electrophoresis
proteomics
