摘要
为研究 STR位点 D2S441的遗传多态性，为法医学应用提供基础数据，应用 PCR及 PAG电泳技术对 260名成都地区汉族无关个体进行了调查，共检出 9个等位基因及 26种基因型，首次获得汉族群体频率分布 ,其等位基因片段大小范围为 131～ 155bp。该位点基因型频率分布符合 Hardy－ Weinberg平衡。家系调查证实了等位基因的传递遵循孟德尔遗传规律。其个人识别能力（ Dp）、杂合度（ H）、多态性信息含量（ PIC）和非父排除率（ PE）分别为 0.9084、 0.7885、 0.7390和 0.5778，表明该位点在法医学个人识别及亲子鉴定中具有较高的实用价值。
Using PCR and PAG, followed by silver staining, the tetrameric STR D2S441 locus was studied in 260 unrelated Chinese individuals living in Chengdu. 9 alleles and 26 genotypes were observed. The range of fragment size was 131bp to 155bp. The genotype distribution of D2S441 locus in Han population was in accordance with Hardy－ Weinberg equilibrium. Family survey confirmed Mendelian inheritance of alleles. The discriminating power (Dp), observed heterozygosity (H), polymorphism information content (PIC) and power of exclusion (PE) were 0.9084, 0.7885, 0.7390 and 0.5778 respectively. The results demonstrated that this locus was highly polymorphic and could be used for forensic identification and paternity testing.
出处
《法医学杂志》
CAS
CSCD
2001年第1期15-16,共2页
Journal of Forensic Medicine
