摘要
目的研究D7S2846、D19S400和D18S535位点在温州汉族人群的遗传多态性。方法EDTA抗凝血样采自194名无血缘关系温州地区汉族个体,用chelex-100法提取DNA,PCR扩增,聚丙烯酰胺凝胶电泳,银染显色分析。结果D7S2846观察到6个等位基因及15种基因型;D19S400观察到10个等位基因及36种基因型;D18S535观察到8个等位基因及26种基因型。各基因座的杂合度(H)分别为:0.644、0.724、0.772;个人识别能力(Dp):0.854、0.940、0.938。结论三个STR基因座具有较高杂合度,等位基因分布符合Hardy-Weinberg平衡,在法医学应用和群体遗传学研究中有较高的价值。
Objective To study genetic polymorphism of D7S2846、D19S400and D18S535in Han population in Wenzhou.Methods DNA was extracted with chelex-100method from EDTA-blood samples of194unrelated individuals in Wenzhou and amplified with PCR technique.The PCR products were analyzed by PAG vertical electrophoresis and silver-stain.Results6alleles and15genotypes of D7S2846、10alleles and36genotypes of D19S400、8alleles and26genotypes of D18S535was observed.The heterozygosities of the three loci are0.644,0.724and0.772;The power of discriminating are0.854,0.940and0.938.Conclusion The heterozygosities of the three loci are high and the frequencies was in accordance with Hardy-Weinberg equilibrium(P>0.05),so the three loci can be used in forensic medicine and in other genetic researches.
出处
《法医学杂志》
CAS
CSCD
2004年第3期160-161,共2页
Journal of Forensic Medicine
