摘要
目的 :了解抑凋亡基因 bcl- 2、抑癌基因 p5 3和癌基因 p2 1基因蛋白在肝细胞癌 (HCC)中的表达状况及意义。方法 :用 bcl- 2、p5 3和 p2 1基因蛋白抗体对 5 5例肝细胞癌合并结节性肝硬变手术标本进行 S- P免疫组化检测 ,分析基因蛋白表达率与病理分级和预后的关系。结果 :bcl- 2基因蛋白在 HCC和结节性肝硬变中的表达率分别为 72 .7% (4 0 /5 5 )和 36 .4% (2 0 /5 5 ) ,两者存在差异 (P<0 .0 5 ) ;p5 3蛋白在 HCC中表达率为 5 4.5 % (30 /5 5 ) ,而肝硬变组织均呈阴性表达 ,两者存在显著差异 (P<0 .0 1) ;p2 1蛋白在 HCC和结节性肝硬变中的表达率分别为 6 3.6 % (35 /5 5 )和 38.2 %(2 1/5 5 ) ,两者无差异 (P>0 .0 5 ) ;而 bcl- 2、p5 3和 p2 1的表达率和表达强度均与 HCC的病理分级无关 (P>0 .0 5 )。另外 ,在随访 2 9例 HCC患者中发现生存率短组 (6个月至 1年 )的 16例中 ,bcl- 2、p5 3和 p2 1的共同表达率为 5 0 % (8/16 )。研究结果提示 :HCC的发生、发展与 bcl- 2、p5 3和 p2 1蛋白的异常表达或突变有关 ;bcl- 2、p5 3和 p2 1之间可能存在相互协同作用 ,对 HCC的预后有一定的影响 ;bcl- 2、p5 3和 p2 1蛋白过度表达反应了 HCC癌细胞的分化程度及增殖情况 ,因而可作为 HCC诊断和预后的参考指标之?
Objective:To study the status and significances of bcl 2,p53 and p21 gene expression in hepatocellular carcinoma(HCC).Methods:The samples resected from 55 cases of hepatocellular carcinoma and nodular liver cirrhosis were detected with the antibodies against bcl 2,p53 and p21 in the S P immunohistochemistry study.The relationships between the expression rate and pathological grades and prognosis were analyzed.Results:The positive expression rate of bcl 2 gene protein in HCC and nodular liver cirrhosis were 72.7%(40/55) and 36.4%(20/55) respectively,both present disparity( P <0.05).The positive expression rate of p53 protein in HCC was 54.5%(30/55),the negative expression of all nodular liver cirrhosis tissues was found,both present striking disparity ( P <0.01).The positive expression rate of p21 protein in the HCC and nodular liver cirrhosis were 63.6%(35/55) and 38.2% (21/55) respectively,both not present disparity ( P >0.05).The expression rate and expression intensity of bcl 2、p53 and p21 was not associated with pathological grades in the HCC ( P >0.05). In addition,together expression rate of bcl 2、p53 and p21 in the follow up patients (16 cases) was 50%(8/16).Conclusion:The results suggest that the occurrence of HCC has a close relation with the over expression and mutation of bcl 2、p53 and p21 gene proteins.There may be a joint action among these genes.Because the expression of bcl 2、p53 and p21 can reflect the differential and proliferative degrees of tumour cells,it can be used as the indicators in the diagnosis and prognosis of HCC.
出处
《华夏医学》
2001年第1期9-11,共3页
Acta Medicinae Sinica
