摘要
目的:观察小蘖碱对氧化型低密度脂蛋白(oxLDL)诱导的大鼠胸主动脉平滑肌细胞(VSMCs)泡沫化及凝集素样氧化型低密度脂蛋白受体1(LOX-1)、核转录因子P65(NF-κB P65)表达的影响。方法:将一部分体外培养的VSMCs分为空白对照组,泡沫化模型组(简称模型组),50μM小蘖碱对照组和50μM小蘖碱干预组,油红O染色后,观察各组VSMCs的泡沫化程度。另一部分体外培养的VSMCs同上分为空白对照组、模型组以及25μM小蘖碱干预组和50μM小蘖碱干预组,预刺激后Western-Blotting检测各组VSMCs的LOX-1蛋白和NF-κB P65蛋白表达水平。结果:空白对照组和小蘖碱组VSMCs未泡沫化,LOX-1和NF-κB P65微量表达。模型组VSMCs中的脂滴聚集成团,形成泡沫化,LOX-1及NF-κB P65的表达较对照组明显增加(P<0.01),小蘖碱干预组泡沫化明显减轻,两小蘖碱干预组VSMCs LOX-1及NF-κB P65蛋白表达较模型组显著降低(P<0.01),高效量组作用更强(P<0.05,P<0.01)。结论:小檗碱可减轻平滑肌细胞源性泡沫细胞化程度,其作用机制可能与抑制NF-κB和LOX-1蛋白表达有关。
Objective：To investigate the effect of berberine on oxLDL induced foam cell formation and lectin-like oxidized low density lipoprotein receptor1（LOX-1）,NF-κB expression in vascular smooth muscle Cells（VSMCs）.Method：VSMC were cultured and classified into four groups：control group,foam cell model group（treated with oxLDL for48hto establish the foam cell models）,or 25uM berberine group and 50uM berberine group（different dose of berberine was pretreated for 2hours）.LOX-1protein expression was detected by western-blot after treated with oxLDL for 48h.Western-blot was applied to detect nuclear expression of NF-κB P65protein after treated with oxLDL for 12h.Results：Compared with the foam cell models,oxLDL agregation and lipid droplets of foam cells in berberine treatment decreased significantly.The LOX-1protein expression decreased at a low ebb after berberine treatment（P〈0.05）.On the other hand,with the increasing concentration of berberine treatment,the LOX-1protein expression decreased progressively（P〈0.01）.With two dosages of berberine,significantly difference was shown between berberine groups and oxLDL group for nuclear NF-κB P65protein expression（P〈0.01）.Conclusion：Berberine inhibits LOX-1and nuclear NF-κB P65protein expression in a dose-dependent manner.This may be part of the mechanism of its anti-atherosclerotic effect.
出处
《微循环学杂志》
2014年第2期31-34,F0004,共5页
Chinese Journal of Microcirculation
