摘要
目的 为中药黄连寻找简便、适用的含量测定方法。方法 用酸性染料比色法和薄层色谱扫描法测定黄连中小蘖碱的含量。结果 小蘖碱的线性范围为 4 15～ 33 2 μg·ml- 1(r =0 9998) ,平均回收率为 98 7% ,RSD为 1 4 0 % (n =5)。结论 五批黄连中小蘖碱的含量测定结果表明两种测定方法具有相同的准确度。因此 。
Objective To find a simple,suitable assay method for berberine in huanglian of Chinese traditional herbs.Methods Berberine in huanglian was determined by acid dye colorimetry and thin Layer chromatography scanning method. Results The determination results of acid dye colorimetry showed that the linear range of berberine was 4.15- 33.2μg/ml ( r =0.9998) and the average recovery was 98.9%,RSD was 1.40%( n =5).Conclusion Determined results of berberine in 5 kinds of huanglian showed the two methods had same accuracy,so acid dye colorimetry may be also used to determine the content of berberine in huanglian.
出处
《兰州医学院学报》
2000年第1期10-11,共2页
Journal of Lanzhou Medical College
