摘要
目的:探讨核转录因子-κB(NF-κB)、巨噬细胞迁移抑制因子(MIF)与子宫内膜异位症(EMS)的临床相关性。方法:采用逆转录聚合酶链反应(RT-PCR)法测定30例非EMS患者在位子宫内膜、68例EMS患者在位和异位子宫内膜的NF-κB和MIF mRNA水平。比较非EMS患者在位子宫内膜和EMS患者的在位子宫内膜及异位子宫内膜的NF-κB和MIF mRNA水平,并且分析EMS患者异位子宫内膜NF-KB、MIF mRNA水平与EMS分期的相关性。结果:①EMS患者异位子宫内膜和非EMS患者在位子宫内膜的NF-KB和MIF mRNA水平差异均具有统计学意义(P<0.01);②不同临床分期EMS患者异位子宫内膜NF-KB和MIF mRNA水平差异均具有统计学意义(P<0.01),并且随着临床分期的增加NF-KB和MIF mRNA水平依次增加(P<0.01);③相关性分析采用Spearman等级相关系数检验显示,EMS患者异位子宫内膜NF-KB与MIF mRNA水平、NF-KB水平与EMS分期、MIF mRNA水平与EMS分期均呈正相关(P<0.01)。结论:核转录因子-κB、巨噬细胞迁移抑制因子在EMS的发生、发展中起重要作用,有可能成为治疗EMS新的靶点。
Objective： To explore the clinical correlation between nuclear factor-κB（ NF-κB）,macrophage migration inhibitory factor（ MIF） and endometriosis. Methods： RT- PCR was used to detect NF- κB and MIF mRNA levels in eutopic endometrium of 30 patients without endometriosis,eutopic endometrium and ectopic endometrium of 68 patients with endometriosis; then the results were compared; the correlation between NF- κB,MIF mRNA levels and endometriosis in ectopic endometrium of patients with endometriosis was analyzed. Results： There were statistically significant differences in NF- κB and MIF mRNA levels between ectopic endometrium of patients with endometriosis and eutopic endometrium of patients without endometriosis（ P〈0. 01）; there were statistically significant differences in NF- κB and MIF mRNA levels among patients with endometriosis of different clinical phases（ P〈0. 01）,NF- κB and MIF mRNA levels increased with clinical stages（ P〈0. 01）; the results of correlation analysis showed that there were positive correlations between NF- KB and MIF mRNA level,NF- KB level and clinical stages of endometriosis,MIF mRNA level and clinical stages of endometriosis,respectively（ P〈0.01）. Conclusion： NF-κB and MIF play important roles in occurrence and development of endometriosis,which probably become to be new treatment targets of endometriosis.
出处
《中国妇幼保健》
CAS
北大核心
2014年第16期2605-2608,共4页
Maternal and Child Health Care of China
