摘要
以从南亚热带水库中分离的拟柱孢藻（Cylindrospermopsis raciborskiiN8）为材料，研究了不同温度（12、16、20、24、28、32℃）、光照强度（6．6、12．4、21．5、30．7、62．9、106．4μmol／（m2·s））和硝态氮浓度（0．5、1、2、4、8、16、32、64、128mg／L）下拟柱孢藻的生长特性．结果表明：在实验设置的温度范围（16～32℃）内拟柱孢藻能够正常生长；最适温度范围为24—28℃，在28℃条件下，具有最大比生长速率，为0．189d^-1；当温度为12℃时，拟柱孢藻叶绿素a浓度一直降低，显著低于其他温度组（16～32℃）．在6．6～106．4μmol／（m2·s）光照强度范围内，拟柱孢藻均呈指数增长趋势，最适光照强度为30．7μmol／（m2·s），其比生长速率达到最大值，为0．156d^-1；高光照强度（62．9～106．4μmol／（m2·s））下拟柱孢藻的比生长速率显著大于低光照强度（6．6—12．4μmol／（m2·s））处理组．拟柱孢藻开始指数增长的最低硝态氮浓度为4mg／L；硝态氮浓度为8mg／L时，拟柱孢藻达到最大比生长速率（O．155d。）；当硝态氮浓度高于16ms／L时比生长速率增加不显著．高硝态氮浓度组（16～128ms／L）拟柱孢藻的叶绿素a浓度和比生长速率显著高于低硝态氮浓度组（0．5—2mg／L）．研究结果说明拟柱孢藻对温度、光照和氮源均有较宽的生态位，有利于在较大空间尺度上进行扩散．
Cylindrospermopsis raciborskii N8 strain was isolated from a sub-tropical reservoir in southern China and cultured with BG-11 culture medium. The effects of temperature, irradiance and nitrate on the growth of this strain were evaluated by a designed experiment with gradients of temperature （ 12, 16, 20, 24, 28, 32℃ ）, irradiance （6.6, 12.4, 21.5, 30.7, 62.9, 106.4 μmol/（m2 · s）） and nitrate （0.5, 1,2, 4, 8, 16, 32, 64, 128 mg/L）. C. raciborskii N8 had normal growth at temperature between 16℃ and 32%, and the maximum specific growth rate （0. 189 d^-1 ） were observed at temperature between 24℃ and 28℃ ; C. raciborskii N8 could not survive at 12℃, and chlorophyll-a concentration was significantly lower than other temperature treatments （ 16 - 32℃ ）. C. raciborskii N8 had a wide adaptation of irradiance ranging from 6.6 panol/（ m2 · s） to 106.4 μmol/ （ m2 · s）. An exponential growth was found from 6.6 to 106.4 μmol/（ m2 · s）. The optimal irradiance was 30.7 μmol/（ m2 · s） and the maximum specific growth rate was 0.156 d^-1. C. raciborskii N8 grew faster significantly in high irradiance conditions （ 62.9 - 106.4 μmol/（ mg·s） ） than that in low irradiance conditions （ 6.6 - 12.4 μmol/（ m2 · s） ） . The species began an ex- ponential growth under nitrate of 4 mg/L, reached the highest specific growth rate （0. 155 d^-1 ） at the nitrate concentration of 8 mg/L. The specifical growth rates became stable when the nitrate concentration exceeded 16 mg/L. C. raciborskii N8 grew faster significantly in the high nitrate conditions （16-128 mg/L） than that in the low nitrate conditions （0.5 -2 mg/L）. The presentexperiments demonstrated that C. raciborskii N8 can adapt for wide temperatures, irradiance and nitrates, and has a potential to colonize and distribute in divers water body over a large scale.
出处
《湖泊科学》
EI
CAS
CSCD
北大核心
2014年第3期441-446,共6页
Journal of Lake Sciences
基金
国家自然科学基金项目(31070416)
暨南大学科研培育与创新基金项目(11612322)
国家级大学生创新创业训练计划项目(1210559020)联合资助
关键词
拟柱孢藻
生长
比生长速率
温度
光照强度
硝酸盐
Cylindrospermopsis raciborskii NS
growth
specific growth rate
temperature
irradiance
nitrate
