摘要
以长白山大花剪秋萝茎节为外植体,对其丛生芽诱导、增殖、生根及保存的最佳培养基进行了筛选。结果表明:最佳茎节诱导培养基为MS+0.8mg/L 6-BA+0.08mg/L NAA,萌发率为90.24%;幼芽最佳增殖培养基为MS+3.0mg/L 6-BA+0.03mg/L NAA,最佳继代时间为25d,平均增殖率为94.1%;最佳生根培养基为1/2MS+1.0mg/L NAA,生根率为87.03%;适合该物种保存的培养基为1/10MS(大量元素)+1/3MS(微量元素)+1/2MS(Fe盐)+1/4MS(有机物质),恢复率86.97%,无明显变异,生长良好。
Taking the internodes of Changbai Lychnis fulgens Fisch. as explants to screen the best medium of their profusion bud induction, proliferation, rooting and preservation. The results showed that the best medium for superior individual internodes induction of the dwarf Lychnis fulgens Fisch. was MS＋0. 8 mg/L 6-BA＋0. 08 rng/L NAA, germination rate was 90. 24% ;the best medium for germ proliferation was MS＋3. 0 mg/L 6-BA＋0. 03 mg/L NAA, propagation coefficient rate was 92. 6% ;the best period was 25 day,for rooting was 1/2 MS＋1. 0 rng/L NAA,rooting rate was 87.03% ;suitable culture medium for the species to save was 1/10MS（major element）＋1/3MS（traee element）＋ 1/2MS（ferric compound）＋1/4MS（organic substance）, recovery rate was 86. 97 %, no obvious variation and well-grown.
出处
《北方园艺》
CAS
北大核心
2014年第10期92-96,共5页
Northern Horticulture
基金
吉林省科技厅科技支撑计划资助项目(20100259)
关键词
大花剪秋萝
茎节培养
物种保存
Lychnis fulgens Fisch.
stem section culture
species preservation
