摘要
橡胶树褐皮接传染结果表明:(1)皮接3个月后,发病率为 29%~50%,被传染感病的砧木植株树皮组织呈现褐色斑或水渍状的病灶,5a后开割割线排胶不正常,割线有不同程度的干涸,症状表现与大田开割树典型的被皮病症状相符。(2)微量沉淀、酶联免疫吸附等方法检测结果表明褐皮病皮接的带菌率为 40%~45%。(3)电镜检查,被传染感病的砧木植株树皮韧皮部筛管细胞内,均可观察到植原体(Phytoplasma.原称MLO),菌体大小为300~850 μm,单位膜厚度为 9~12μm,但健康对照树未发现有上述菌体存在。
Test on transmission of brown bast of rubber tree by bart-grafting revealed that typical brown bast symptom appeared on the trunks of the Originally healthy stocks three months after grafting. The disease incidence was 29-50 %. The symptom was water-soaked with brown lesions on the bark, dryness on the cut and reduced latex flow. Micro- precipitation and ELISA showed that trunks with brown bast accounted for 40-45 % in the origi nally stocks 13-14 months after grafing. When examined under electronic microscope, phy- toplasma were discovered in the cells of the phloem tissues from the trunks of the originally healthy stocks. The size of the phytoplasma was 300-850 um, with one layer of membrane of 9~12 um thick. No phytoplasma was discovered in the cell of the phloem tissues from the controlled trunks (healthy stocks, not grafted with diseased scions).
出处
《热带作物学报》
CSCD
2000年第3期15-20,共6页
Chinese Journal of Tropical Crops
关键词
橡胶树褐皮病
病皮
皮接传染
流行病学
brown bast of rubber tree diseased bark transmission by bark-grafting