摘要
目的 建立尼莫地平脂肪乳注射液的高效液相色谱分析方法,并应用于大鼠体内的药动学研究.方法 血浆样品以乙醚-正己烷（体积比1∶1）萃取,采用HPLC法进行测定分析.色谱柱为安捷伦XDB-C18柱（250 mm×4.6 mm,5μm）;流动相为甲醇-水（体积比为65∶35）;流速为1.0 mL·min^-1;检测波长为360 nm.结果 尼莫地平在0.1～40μg·mL-1呈现良好的线性关系（r^2=0.999 9）,方法的日内、日间精密度RSD值分别在0.62％～8.8％和1.6％ ～ 8.4％之间,相对回收率为85.5％～ 103.2％,绝对回收率为78.5％～91.6％,定量下限为0.1·m^L-1.大鼠注射给予尼莫地平脂肪乳注射液（2.5 mg·kg^-1）后,t1/2（β）为（19.91±4.11） min,AUC0-120为（138.63±35.70） mg·min·L-1.结论 本方法准确、简便、灵敏,可用于尼莫地平脂肪乳注射液在大鼠体内的药动学测定.
Objective To establish a HPLC method for the determination of nimodipine fat emulsion injection in rat plasma, and to investigate its pharmacokinetics. Methods Plasma samples were analyzed by HPLC after liquid extraction of ether and n-hexane （ 1：1 ）.The chromatographic conditions were as follows： Agilent XDB- C 18 chromatographic column; the mobile phase was methanol：water （65：35）; the flow rate was 1.0 mL · min^-1; UV detection wavelength was 360 nm. Results Nimodipine had a good linear relationship when its concentration was between 0.1-40 μg · mL^-1 .The intra-day and inter-day precision of this method were 0.62% -8.8% and 1.6%-8.4%.Relative recoveries and absolute recoveries were between 85.5%-103.2% and 78.5% -91.6%.Lower limit of quantification of nimodipine was 0.1 μg · mL^-1 .Rat plasma samples were collected after a single dose of nimodipine fat emulsion injection and pharmacokinetic parameters of nimodipine were estimated,respectively.The t1/2（β） were （19.91±4.11） min and the AUCo_12o values were （138.63±35.70） mg · min · L^-1 .Conclusion The method is accurate, simple and sensitive, which can be used for the determination of serum nimodipine concentration.
出处
《广东药学院学报》
CAS
2014年第2期146-149,共4页
Academic Journal of Guangdong College of Pharmacy
基金
广东省科技计划项目(2011B031800049)
广东省教育部产学研结合项目(2011B090400164)
广州市重大科技计划项目(201300000046)
