摘要
目的建立一种离子色谱-积分脉冲安培法测定燕窝、速食燕窝及银耳、海藻酸钠、鱼胶等常见伪品燕窝中N-乙酰神经氨酸的含量。方法采用DionexICS-5000离子色谱仪、DionexASRSRR5004-mm抑制器、电导检测器测定，色谱柱为CarbonPacPAI柱，柱温为30℃．流动相为100mmol／LNaOH＋50mmol／LNaAc，流速为1．0mL／min，进样量为10μL，分别测定燕窝、速食燕窝及伪品中N-乙酰神经氨酸的含量。结果N-乙酰神经氨酸在0．25～5．00μg/mL范围内线性良好，r=0．9999；在燕窝中的平均回收率为99．7％，RSD为0．82％（n=6）。结论本方法灵敏度高、重复性好，可准确测定燕窝中^L乙酰神经氨酸的含量，为燕窝的质量控制提供了一种检测方法。
Objective To establish a method for the determination of N-acetyl neuraminic acid in bird＇s nests,its fast foods and common counterfeit products. Methods The Dionex ICS-5000 ion chromatography was adapted and equipped with CarbonPac PAI analytical column, eluted by 100 mmoL/L of NaOH＋50 mmol/L NaAc solution at a flow of 1.0 mL/min. Each injection volume was 10 μL with Dionex ASRSRR500 4-mm suppressor and the conductive detector. Results It showed a good linearity in the concentration range of 0.25 and 5.00 μg/mL with r of 0.999 9. The mean recovery was 99. 7% with RSD of 0. 82% （ n = 6）. Conclusion This method was highly sensitive with good repeatability and accuracy, which is suitable for the determination of N-acetyl neuraminic acid content in the bird＇s nest.
出处
《广东药学院学报》
CAS
2014年第1期40-43,共4页
Academic Journal of Guangdong College of Pharmacy
基金
国家自然科学基金面上项目(81173498)
广东省高等学校珠江学者岗位计划资助项目(2012年度)
