摘要
目的:探讨RGC32与转化生长因子β1(TGF-β1)在肺腺癌中表达的相关性,明确二者之间调控关系及其对肺腺癌细胞增殖的影响。方法:Real-time PCR检测52例肺腺癌及相应癌旁组织中RGC32与TGF-β1的表达;应用TGF-β1受体特异性抑制剂SB-431542处理A549细胞,检测处理后细胞中RGC32基因的表达,并用MTT法测定细胞增殖活力,双标流式细胞术检测细胞凋亡。结果:RGC32和TGF-β1在肺腺癌组织中表达均呈现显著增加,且二者呈正相关;SB-431542处理后,RGC32蛋白表达显著下调,A549细胞的凋亡增加,增殖活力降低。结论:RGC32和TGF-β1在肺腺癌中均呈现表达上调,二者呈正相关。RGC32是TGF-β1信号通路下游重要的调控基因,能够参与肺腺癌细胞增殖能力的调节。
Objective：To research the relationshiP between RGC32 and TGF - β1 in lung adenocarcinoma,and the regulation between them,and their influence to Proliferation of A549 cells. Methods：All 52 cases of lung adeno-carcinoma and Peri - cancerous tissues were selected to detect the exPression of RGC32 and TGF - β1 by Real - time PCR. RGC32 was treated by SB - 431542（sPecific inhibitor of TGF - β1 recePtor）in A549 cell,the exPression of RGC32 was detected by Real - time PCR,the Proliferation of the treated cells was detected with MTT,the aPoPtosis was detected by flow cytometry. Results：ComPared with Peri - cancerous tissues,the exPression of RGC32 and TGF -β1 Presented an obvious uP - regulation in lung adenocarcinoma;there was a Positive relation between them. After treated with SB - 431542,the exPression of RGC32 decreased,the aPoPtosis increased significantly,and the Prolifera-tion decreased significantly. Conclusion：The exPression of RGC32 and TGF - β1 gene Presente an obvious uP - regu-lation in lung adenocarcinoma,there is a Positive relation between them. RGC32 is an imPortant downstream gene of TGF - β1 signal Pathway,and can regulate the Proliferation of lung adenocarcinoma cells.
出处
《现代肿瘤医学》
CAS
2014年第5期1009-1011,共3页
Journal of Modern Oncology
基金
辽宁省博士科研启动基金项目(编号:20091108)
