摘要
目的了解温州医科大学附属第一医院分离的鲍曼不动杆菌的耐药情况;应用RAPD技术监测鲍曼不动杆菌医院感染状况,探讨鲍曼不动杆菌分子流行病学特点。方法应用纸片扩散法对100株鲍曼不动杆菌进行药物的敏感性实验;应用RAPD DNA基因分型方法对100株鲍曼不动杆菌进行基因分型,通过NTSYSpc软件绘制菌株之间亲缘关系树状图,统计各型别在该院临床各科室分布情况。结果分离的鲍曼不动杆菌除对头孢哌酮/舒巴坦,左旋氧氟沙星等抗生素保持相对较高的敏感性以外,对其它常见抗生素的耐药率均已超过了60%;将100株鲍曼不动杆菌用RAPD法分为A-F 6个型别,其中B型与C型之间的同源关系最近。鲍曼不动杆菌以6个型别中的A型和B型流行为主,其中A型28株,B型24株。结论该院鲍曼不动杆菌耐药情况相当严重,以A型和B型为主要流行株,个别科室存在着A-F型的科内小流行。
Objective To understand the antimicrobial resistance of Acinetobacter baumannii isoaltes in the First Affiliated Hospital of Wenzhou Medical University,to monitor the nosocomial infection of Acinetobacter baumannii by random amplified polymorphic DNA（ RAPD） technology and to study the molecular epidemiological characteristics of Acinetobacter baumannii. Methods The disk diffusion method was applied to test the susceptibility of 100 Acinetobacter baumannii strains. Random amplified polymorphic DNA was used to determine the homology of these isolates. Phylogenetic tree was drew by NTSYSpc software to study the distribution of Acinetobacter baumannii in each department. Results In addition to cefoperazone/ sulbactam and levofloxacin,these Acinetobacter baumannii isoaltes showed drug resistance above 60% to other common antibotica. The 100 Acinetobacter baumannii strains can be divided into six genotypes,A,B,C,D,E and F in the light of the number and size of DNA fragment,in which B and C had the closest phylogenetic relationship. The most popular genotypes in the hospital were A and B, 28 isolates belonged to A and 24 isolates belonged to B. Conclusion The antimicrobial resistance of Acinetobacter baumannii is very serious in the hospital,with A and B as the popular genotypes. Some departments are found small-scale epidemic of the 6 genotypes of Acinetobacter baumannii.
出处
《中国卫生检验杂志》
北大核心
2014年第8期1188-1190,共3页
Chinese Journal of Health Laboratory Technology
关键词
鲍曼不动杆菌
耐药性
RAPD
DNA同源性
亲缘关系
Acinetobacter baumannii
Drug resistance
RAPD
DNA homology
Phylogenetic relationship
