摘要
目的:观察通脉化浊汤(TMHZ)对冠心病模型大鼠肝脏ApoAⅠ、B mRNA和蛋白表达水平的影响,探讨其治疗冠心病分子机制。方法:66只清洁级SD大鼠按体质量和性别随机分为正常组,模型组,血脂康(XZK)组,TMHZ高、中、低剂量组,除正常组外,运用钙超载结合脂质浸润法建立冠心病大鼠模型,并用Ⅱ导心电图检验造模成功。连续灌胃给药4周后,将各组大鼠常规处死并提取组织。用RT-PCR和Western Blot法检测各组肝脏ApoAⅠ、B mRNA和蛋白表达量,分析其表达差异。结果:①模型组ApoAⅠmRNA和蛋白表达水平较正常组明显降低(P<0.05),TMHZ各治疗组及XZK组与模型组比较ApoAⅠmRNA和蛋白表达水平明显升高(P<0.05,P<0.01)。②模型组ApoB mRNA和蛋白表达水平较正常组明显升高(P<0.05,P<0.01),TMHZ各治疗组及XZK组与模型组比较ApoB mRNA和蛋白表达水平明显降低(P<0.01,P<0.05)。结论:TMHZ能够在基因和蛋白水平提高或稳定ApoAⅠ的表达,降低或抑制ApoB的表达,从而增加血浆中ApoAⅠ,降低ApoB的含量,这可能是其临床应用治疗冠心病,降低血脂,延缓动脉粥样硬化进程的分子机制。
Objective： To observe the effect of Tongmai Huazhuo Decoction （TMHZ） on mRNA and protein expression of ApoAI, B in the liver of model rat with coronary heart disease, and to explore its molecular mechanism in treatment of coronary heart disease. Methods： Totally 66 clean grade SD rats were randomly divided into a normal group, model group, Xuexhikang （XZK） group, TMHZ high, medium and low dose group by weight and sex. Except for the normal group, rat models with coronary heart disease were established by using lipid infiltration calcium overload, and the lead II ECG of the rats was recorded to test whether it was successful. After intragastric administration for 4 weeks, the rats in each group were routinely sacrificed to extract tissues. The liver mRNA and protein expression of ApoAI, B were measured by RT-PCR and Western Blot, and then their expression differences were analyzed. Results： （~）The mRNA and protein expression levels of ApoAI in the model group were obviously lower than those in the normal group （P〈0.05）. Compared with the model group, mRNA and protein expression levels of ApoAI in each TMHZ treatment group and XZK group were significantly increased （P〈0.05, P〈0.01）. （1）The mRNA and protein expression levels of ApoB in the model group were remarkably higher than those of the normal group （P〈0.05, P〈0.01）, mRNA and protein expression levels of ApoB in each TMHZ treatment group and XZK group were significantly lower than those of the model group （P〈0.01, P〈0.05）. Conclusion： TMHZ is able to increase or stabilize ApoAI expression, and decrease or inhibit ApoB expression in gene and protein levels, thus increase the content of ApoAI and lower ApoB in plasma, which may be its molecular mechanisms to treat coronary heart disease, lower blood lipids, and delay the process of atherosclerosis.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2014年第5期1470-1476,共7页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
National Key Discipline of State Administration of Traditional Chinese Medicine during the‘Twelfth Five-year’Plan Period(TCM Collateral Disease Project)
Liaoning Province Key Medical Project(No.200822)
Project of Liaoning Provincial Department of Education(No.2008455)~~
