摘要
目的 探讨冷冻法构建大鼠角膜内皮功能不全模型的可行性及B4G12细胞的生物安全性和泵功能.方法 实验研究.用随机数字表法将30只SD大鼠随机分为单纯冷冻组及冷冻＋B4G12细胞移植组;液氮冷冻法制备角膜内皮功能不全模型,前房注射法将B4G12细胞移植到模型动物眼内.术后定期对各组动物进行眼前节观察、激光共聚焦显微镜检察以及病理学检查.结果 冷冻后角膜内皮细胞被完全去除,单纯冷冻组大鼠角膜水肿、混浊,B4G12细胞移植组术后2周角膜均恢复透明,厚度与正常角膜接近.激光共聚焦显微镜及病理染色证实单纯冷冻组后弹力层裸露,基质水肿;细胞移植组内皮细胞贴附良好,内皮完整.结论 液氮冷冻法可用于制备大鼠角膜内皮功能不全模型,B4G12细胞移植后短期不引起异常新生物及炎症反应,具有内皮泵功能.
Objective To set up an animal model of corneal endothelium deficiency and test the bio-safety and pump function of B4G12 cells with this model.Methods Thirty SD rats were divided into cryo-injury group and cryo-injury with B4G12 cell transplantation group.Models of corneal endothelium deficiency were created by cryo-injury with liquid nitrogen,and then B4G12 cells were transplanted into the eyes by anterior chamber injection.Corneas were checked under slit lamp and confocal microscope observations at some specific time points,pathological staining was also performed.Results The corneal endothelial cells were removed completely from the Descemet＇s membrane after cryo-injury.Cells transplanted firmly stuck to the Descemet＇s membrane.The corneas in the cryo-injury group were swelling with haze,while in the transplantation groups,the corneas restored transparence and normal thickness 2 weeks after the surgery.Confocal microscope and HE staining confirmed that in the cryo-injury group,the Descemet＇s membrane was denuded and the cornea stroma layer was in edema,on the contrary,in the other group,the transplanted cells completely covered the Descemet＇s membrane.Conclusion Cryo-injury can be used for building the rat model of corneal endothelium deficiency,no neoplasm and inflammation reaction were found during the observation after B4G12 cells transplanted and B4G12 cells had the pump function in vivo.
出处
《中华眼科杂志》
CAS
CSCD
北大核心
2014年第4期273-276,共4页
Chinese Journal of Ophthalmology
关键词
角膜疾病
内皮
角膜
细胞移植
冷冻
疾病模型
动物
Corneal diseases
Endothelium, corneal
Cell transplantation
Freezing
Disease models, animal
