摘要
对耐热高产菌株枯草芽孢杆菌GXA-28分批发酵生产γ-聚谷氨酸的动力学特征进行了研究,基于菌体生长特性,结合Logistic方程和Luedeking-Piret方程,提出了菌体生长、产物合成、葡萄糖消耗以及谷氨酸钠消耗的动力学模型。应用Origin8.5对数据进行分析处理,得到了Bacillis subtilis GXA-28分批发酵合成γ-聚谷氨酸相应的动力学参数。将模型预测值和实验值进行比较,结果表明,模型基本反映了枯草芽孢杆菌GXA-28分批发酵过程中的动力学特征。
The kinetics feature of a heat-resistant and high-yield γ-polyglutamic acid strain Bacillis subtilis GXA-28 was investigated. Based on bacterial growth characteristics, combining with Logistic and Luedeking-Piret equation, the kinetic model of microbial growth, γ-polyglutamic acid formation and glucose consumption during fermentation were proposed. The data was analyzed with origin 8.5 to generate the kinetics parameters of γ-ploy glutamic acid production by B. subtilis GXA-28 batch fermentation. The prediction value was compared with the experimental value; it showed that the model could provide reasonable description for the batch fermentation process of B. subtilis GXA-28.
出处
《中国酿造》
CAS
2014年第4期75-78,共4页
China Brewing
基金
广西研究生教育创新计划资助项目(YCBZ2012004)
2013年广西大学亚热带农业生物资源保护与利用国家重点实验室研究课题
2013年广西大学微生物及植物遗传工程教育部重点实验室主任基金课题
