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电针对化合物48/80致小鼠皮肤瘙痒模型的影响及与TRPV1的相关性研究 被引量：4

Effect of electroacupuncture at Quchi(LI11) or Xuehai(SP10) on compound 48 /80-induced scratching behavior in mice
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摘要 目的观察化合物48/80(Compound 48/80,C48/80)对正常和TRPV1敲除小鼠诱发的瘙痒反应及电针不同穴位对其的影响,明确针灸止痒效应及与TRPV1的关系。方法选用野生型C57BL/6小鼠(WT小鼠)和TRPV1敲除小鼠(KO小鼠)各30只,分为模型组、曲池组、血海组,连续两日分别于其颈部中央皮下注射C48/80,曲池组、血海组于第二日注射前给予电针治疗30min,观察电针干预前后瘙痒行为即搔痒次数/30min的变化。结果 C48/80能诱发WT小鼠和KO小鼠对注射处显著的搔抓反应,模型组WT小鼠第一日的搔痒次数为(180.69±79.00)次/30min,KO小鼠第一日的搔痒次数为(124.87±31.52)次/30min,二者差异具有统计学意义(P<0.05)。电针曲池、血海均能有效抑制此种搔抓反应(P<0.01,P<0.05),曲池组WT小鼠的电针前后变化率为49.66%,KO小鼠为35.66%,二者差异具有统计学意义(P<0.01);血海组WT小鼠为52.72%,KO小鼠为34.91%,二者差异具有统计学意义(P<0.01)。而对WT与KO小鼠而言,穴位间止痒效应的差异无统计学意义(P>0.05)。结论 TRPV1参与C48/80诱发的瘙痒反应的产生,电针能明显抑制化合物48/80诱发的小鼠皮肤瘙痒反应,且TRPV1参与针刺效应的产生。 Objective To observe the effect of electroacupuncture （EA） at Quchi （LI11 ） or Xuehai （SP10） on experimental cutaneous pruritus induced by compound 48/80 in mice. Methods 30 BALB/c mice were randomly divided into model group, Quchi group and Xuehai group. Compound 48/80 was injected subcutaueously into the rostral part of the back in two consecutive days. Quchi group and Xuehai group were given electro - acupuncture treatment for 30min before injection of the second day. The number of scratches was counted for 40 min after injection of compound 48/80. Itching behavior was observed before and after the intervention of the electro - acupuncture. Results The scratching reaction can be significantly induced in mice after the injection of compound 48/80. Acupuncture at Quchi（LI11 ） and Xuehai （SP10） caused a potent depressant effect on compound 48/80 - induced scratching behavior. However there was no significant difference between Quchi （ LI11 ） group and Xuehai group. Con- clusion EA can significantly inhibit skin itching reaction in mice induced by compound 48/80, and may be one of the effective methods for treatment of pruritus. The selection of acupoint is the important factor affecting antipruritic effect of EA.
作者 梁超 王媛 徐斌 余芝
机构地区 南京中医药大学国家重点省部共建针药结合实验室
出处 《时珍国医国药》 CAS CSCD 北大核心 2014年第4期987-989,共3页 Lishizhen Medicine and Materia Medica Research
基金 国家重点基础研究发展计划"973"资助(No.2011CB505206) 国家自然科学基金(No.81202744) 江苏省高校自然科学研究面上项目(No.11KJB360008) 江苏省特色优势学科资助项目 江苏省高校青蓝工程优秀科技创新团队资助项目
关键词 瘙痒 电针 TRPV1 C48 80 Itch Electroacupuncture TRPV1 C48/80
分类号 R245.97 [医药卫生—针灸推拿学]
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参考文献12

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二级参考文献29

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共引文献57

同被引文献60

  • 1刘卫英,邓元江,刘宝莲.梅花针叩刺配合养血定风汤加味治疗局限性神经性皮炎47例疗效观察[J].新中医,2005,37(3):54-55. 被引量：2
  • 2罗明富,何俊娜,郭莹,李翠红,张金铃.电针和悬灸对“大椎”穴区肥大细胞脱颗粒不同影响的研究[J].针刺研究,2007,32(5):327-329. 被引量：27
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时珍国医国药
2014年 第4期

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