摘要
目的:探讨中药注射剂引起RBL-2H3细胞脱颗粒情况及其机制。方法:RBL-2H3细胞分别与阳性药物C48/80及15种中药注射剂作用,从形态学和超微结构观察、β-氨基己糖苷酶释放检测等方面研究RBL-2H3细胞脱颗粒情况。用WST-8法检测中药注射剂对RBL-2H3细胞和BRL细胞的细胞毒作用。结果:甲苯胺蓝染色和电镜观察细胞超微结构变化从形态学上证明中药注射剂引起RBL-2H3细胞脱颗粒。上清中β-氨基己糖苷酶释放率检测表明脉络宁、清开灵、参麦和痰热清注射液明显促进酶的释放,酶释放率在20%以上,与阴性对照组比较差异有显著性意义(P<0.05)。进一步的研究发现,痰热清注射液成分中的黄芩和生脉注射液成分中的红参、五味子与RBL-2H3细胞的脱颗粒有关。大部分中药注射剂致RBL-2H3细胞脱颗粒的作用与细胞毒作用相关,但有的中药注射剂致脱颗粒无细胞毒作用。结论:一些中药注射剂可致RBL-2H3细胞脱颗粒,中药注射剂中有的成分与RBL-2H3细胞脱颗粒相关;有的中药注射剂致RBL-2H3细胞脱颗粒与细胞毒作用有关,而pH和渗透压可影响细胞的脱颗粒。
Objective： To study the degranulation of RBL-2H3 ceils induced by Chinese medicinal injections and its mechanisms. Methods： RBL-2H3 ceils were interacted with C48/80 and 15 kinds of Chinese medicinal injections, and research the degranulation of RBL-2H3 ceils through morphological and uhra-structure observation and β-hexosaminidase activity detection. The cytotoxicity effect of Chinese medicinal injections on both RBL-2H3 and BRL cells was measured by WST-8 assay. Results： Toluidine blue staining and ultra-structure of electronic microscope observation morphologically proved that degranulation of RBL- 2H3 ceils was induced by Chinese medicinal injections. The release rate of β-hexosaminidase in superuatant showed that Mailuoning Injection, Qingkailing Injection, Shenmai Injection, and Tanreqing Injection had the positive effect on releasing of enzyme, and with release rate over 20%, there was a statistically significant difference from negative control group （P 〈0.05 ）. The further study showed that Radix Scutellariae in Tanreqing Injection and Radix Ginseng Rubra and Fructus Schisandrae Chinensis in Shengmai Injection were responsible for the granulation of RBL-2H3 ceils. Most of the Chinese medicinal injections induced degranulation had cytotoxicity effect on RBL-2H3 cells, but some Chinese medicinal injections induced degranulation had no cytotoxicity effect. Conclusion： Some Chinese medicinal injections can induce degranulation of RBL-2H ceils; the ingredients in Chinese medicinal injections are responsible for the degranulation. The mechanism of degranulation has relationship with cytotoxicity. The pH value and osmotic pressure can influence degranulation.
出处
《上海中医药大学学报》
CAS
2012年第5期85-91,共7页
Academic Journal of Shanghai University of Traditional Chinese Medicine
基金
国家科技部重大新药创制基金资助项目(2009ZX09502-002)
