摘要
以洋葱表皮细胞为材料,探究其经卡诺氏液固定后进行细胞骨架制片观察时,1%Triton X-100抽提非骨架蛋白的最佳时间,并研究洋葱不同表皮细胞及经不同理化因素处理的材料,经卡诺氏液固定后进行细胞骨架制片的步骤及制片效果。结果表明:经卡诺氏液固定后,1%Triton X-100抽提非骨架蛋白内表皮30min较佳,外表皮25min较佳;不同成熟度的鳞片叶细胞骨架形态及稳定性有差异;经紫外线、温度、秋水仙素处理后增加固定步骤再制片对制片观察结果基本无影响。
Optimal time of extracting non--cytoskeleton protein and the effect of dipping in carnoys fixative were studied. The material was pre--immobilized onion inside and outside epidermial cells. The cytoskeleton of epidermial cell from different places in the pre-- immobilized onion and pre-- immobilized onion inner-- epidermial cell treated with different temperatures, UV--B radiation and colchicine were researched. Results showed that extracting non--cytoskeleton protein with 1% Triton X--100 for 30min had a positive effect on the microscopic observation on cytoskeleton of the pre- immobilized onion inner--epidermal cell and for 25min in outer skin. Different maturity of scale leaves had an influence on cytoskeleton morphology and stability. Carnoys fixative has no effect on the cytoskeleton.
出处
《龙岩学院学报》
2014年第2期23-28,共6页
Journal of Longyan University
关键词
洋葱表皮
细胞骨架
卡诺氏
紫外线
温度
秋水仙素
onion skin； cytoskeleton； Carnoys fixative； UV-- B radiation； temperature
colchicine
