摘要
以山东省枣庄市峄城区中国石榴种质资源圃内38个石榴品种为试材，用20条RAPD引物对其进行扩增，根据扩增结果进行UPGMA聚类分析和主坐标分析，研究了38份材料的亲缘关系。结果表明：20条RAPD引物在38份材料中共产生146个位点，多态性位点为109，多态性比率为74．6％；38个品种间遗传相似系数变化范围为0．45～1．00；在相似系数为0．58处，将38个品种可以分为四大类，得出品种间的亲缘关系与形态学和地理分布没有明显相关性；38份材料可以分为两大类，主坐标分析结果与UPGMA的聚类结果存在一定差异。
Taking 38 pomegranate cultivars from China germplasm nursery at Yicheng of Zaozhuang as materials, they were amplified by 20 primers of RAPD, and the cluster analysis and principal coordnate analysis based on amplification results,the genetic relationship of 38 culfivars of the material were studied. The results showed that total 146 bands were amplified from 38 cultivars,in which 109 bands were polymorphie. The percentage of polymorphic band was 74. 6 %. The genetic similarities coefficient between cultivars ranged from 0. 45 to 1.0 pomegranate cultivars were divided into four groups at 0. 58 based on UPGMA method. The clustering analysis results did not show obvious correlation with the morphologic classification and the geographical distribution. 38 pomegranate cultivars were divided into two groups based on the principal coordinates analysis, the results of the principal coordinates analysis were not consistent with that of the UPGMA cluster.
出处
《北方园艺》
CAS
北大核心
2014年第9期101-105,共5页
Northern Horticulture
基金
国家林业公益性行业科研专项资助项目(201204402)
国家林业局林木种苗工程资助项目([2012]1888号)
枣庄学院博士启动基金资助项目(307070903)
枣庄学院科研基金资助项目(307050907)
枣庄市石榴工程技术研究中心建设资助项目
关键词
石榴种质资源
RAPD
遗传关系
主坐标分析
pomegranate germplasm
RAPD
genetic relationship
principal coordinates analysis
