摘要
抗体是现代生命科学研究中极其重要的工具,小分子抗体技术在中医药研究领域具有广阔的应用前景。结合创新团队开展的研究工作,介绍了中药小分子单克隆抗体技术平台建立的背景和抗体制备的技术难点,针对基于中药小分子单克隆抗体的技术产品,如ELISA检测试剂盒、免疫亲和色谱柱、胶体金试纸、荧光标记抗体和抗体芯片等,探索实践了其在中医药研究领域中的多种应用,并对中药小分子单克隆抗体技术的科学意义和应用价值进行了展望。
Antibody is a tool of vital importance in modem bioscience research, small molecular antibody technology has a broad application prospect in the field of receptor binding analysis, enzyme assays, and quantitative and/or qualitative analytical techniques of Chinese materia medica （CMM） research. In this paper, combining with the research work carried out by our innovation team, we introduced the establishment background of the small molecular monoclonal antibody technology platform in CMM and technical difficulties in antibody preparation. In view of the technology products based on small molecular monoclonal antibody of CMM, such as ELISA test kit, immune affinity chromatography column, colloidal gold test paper, fluorescently labeled antibodies, and antibody microarrays, we explored and practised the various applications based on the small molecular monoclonal antibody technology looking forward to its scientific significances and application values in the field of CMM research.
出处
《中草药》
CAS
CSCD
北大核心
2014年第7期895-899,共5页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(81274043
81373542)
关键词
中药小分子
单克隆抗体制备
酶联免疫吸附法
免疫亲和色谱柱
胶体金试纸
荧光标记抗体
抗体芯片
small molecules of Chinese materia medica
monoclonal antibody preparation
enzyme-linked immunosorbent method
immune affinity chromatographic column
colloidal gold test paper
fluorescenty-labeled antibodies
antibody microarrays
