摘要
为实现自然水细菌总数快速检测的需求，基于三磷酸腺苷（ATP）生物发光法，研制了K2010型水质细菌总数快速检测仪。采用研制的快速检测仪分别对大肠杆菌标准品和太湖水实际样品进行了检测。此检测仪集成高精度AD转换器件，以小型光电倍增管（PMT）为光电转换器件，通过合理的电路、光路设计，结合实验室的自制试剂，实现了对自然水环境中细菌总数的快速检测；并可以通过GPS模块进行定位，将位置信息和检测结果通过GPRS模块传到远程终端。对大肠杆菌标准品进行检测的结果表明，在4．6×101～4．67×107CFU／mL范围内，相关性达到0．9515，检测下限达到46CFU／mL，满足水质细菌总数检测需求。针对自然水环境检测的需求，对3种不同的水处理方法进行了比较分析，在此基础上分别在夏季和冬季对太湖水进行了采样和检测。结果表明，夏季，采集到23个样本，与国标法对比发现，在102—105CFU／mL浓度范围内两者相关系数R一0．9169；冬季采集到40个样本，水中细菌浓度较低（在101～104CFU／mL之间）。检测结果说明，研制的K2010检测仪能正确区分富营养水质和贫、中营养水质，且检测速度快，准确度较高，重复性良好，可在水环境监测中用于水质细菌的快速筛查。
An optical instrument for rapid detection of bacteria from natural water is presented in this pa per,which is based on the principle of adenosine triphosphate （ATP） bioluminescence reaction. The E. coli and real samples from Taihu Lake were tested by the optical instrument, and results were compared with TBC results provided by plate counting. The instrument integrated with high-precision AD converter and a small photomultiplier tube （PMT） module is used to improve the optical detection sensitivity. Combined with homemade ATP bioluminescence biosensors, this instrument can be used for rapid detection of bacteria. An calibration curve is obtained from the bioluminescence intensity for E. coli in the range of 4.6 ×101 CFU/mL-4.67 ×107 CFU/mL with a good orrelation coefficient of 0. 951 5. Three methods of sample treatment were compared in order to test bacteria from natural water. Then, 23 samples and 40 samples were collected and tested in summer and winter, respectively, from Taihu Lake, Wuxi of China. In summer collection, a linear relationship between RLU and TBC provided by plate counting is obtained with correlation coefficient of 0. 916 9. In winter collection, the results of TBC by K2010 show that 40 samples are all mesotrophic, consistent with plate counting method, which indicates that 38 samples are mesotrophic and 2 samples are oligotrophic. In summary,this system can complete aprocess of detection in a few minutes,and with high sensitivity and reproducibility,so it has potential in many fields,such as food safety,environmental moniloring,and water pollution forecast.
出处
《光电子．激光》
EI
CAS
CSCD
北大核心
2014年第4期664-668,共5页
Journal of Optoelectronics·Laser
基金
国家重点基础研究发展计划(2011CB933202)
国家自然科学基金(61027001,61002037)
中国科学院战略性先导专项“面向感知中国的新一代信息技术研究”(XDA06020101)资助项目
关键词
细菌总数
生物发光
快速检测
三磷酸腺苷(ATP)
toral bacteria count
bioluminescence
rapid detection
adenosine triphosphate （ ATP ）
