白细胞相关免疫球蛋白样受体-1在免疫性血小板减少症患儿中的表达被引量：3

Expression of leukocyte-associated Ig-like receptor-1 in children with immune thrombocytopenia

导出

摘要目的观察白细胞相关免疫球蛋白样受体-1(LAIR-1)在免疫性血小板减少症(ITP)患儿中的表达变化,探讨其在ITP发病中可能的机制。方法采用流式细胞术检测40例ITP患儿外周血CD4+T细胞、CD8+T细胞及CD19+CD20+B细胞膜LAIR-1的表达率;ELISA检测ITP患儿血清中可溶性LAIR-1(sLAIR-1)含量及实时荧光定量PCR法检测ITP患儿LAIR-1 mRNA表达水平。32名同龄健康儿童作为对照组。结果 ITP组CD19+CD20+B细胞比例高于对照组(P<0.05),而CD4+T细胞比例低于对照组(P<0.05)。ITP组CD4+T细胞、CD8+T细胞膜LAIR-1的表达低于对照组(P<0.05)。ITP组血清sLAIR-1含量和LAIR-1 mRNA表达高于对照组(P<0.05)。结论 ITP患儿中CD4+T细胞、CD8+T细胞膜上LAIR-1表达降低,血清sLAIR-1表达增高,提示LAIR-1可能是导致ITP患儿免疫失衡的重要因素。 Objective To study the expression of leukocyte-associated Ig-like receptor-l（LAIR-1） in children with immune thrombocytopenia （ITP）, in order to explore the possible role of LAIR-1 in the pathogenesis of childhood ITP. Methods Expression levels of LAIR-1 on CD4＋ T cells, CD8＋ T cells and CD19＋CD20＋ B cells of peripheral blood were measured in 40 children with ITP by flow cytometry. Serum level of solubility LAIR-1 （sLAIR-1） was measured using ELISA. Real-time PCR was used to measure LAIR-1 mRNA expression. Thirty-two healthy children served as the control group. Results The percentages of CD19＋CD20＋ B cells in the ITP group were significantly higher than in the control group （P〈0.05）. In contrast, the percentage of CD4＋ T cells in the ITP group was significantly lower than in the control group （P〈0.05）. The expression levels of LAIR-1 on CD4＋ T cells and CD8＋ T cells were significantly lower in the ITP group than in the control group （P〈0.05）. Serum sLAIR-1 level and LAIR-1 mRNA expression in the ITP group significantly increased compared with the control group （P〈0.05）. Conclusions LAIR-1 expression on CD4＋ and CD8＋ T cells decreases and serum sLAIR-1 level increases in children with ITP, suggesting that LAIR-1 may play an important role in immune imbalance in these children.

作者黄美玲李长钢王国兵祖莹

机构地区合肥医学院深圳儿童医院血液科

出处《中国当代儿科杂志》 CAS CSCD 北大核心 2014年第4期370-374,共5页 Chinese Journal of Contemporary Pediatrics

关键词免疫性血小板减少症白细胞相关免疫球蛋白样受体-1 免疫失衡儿童 Immune thrombocytopenia Leukocyte-associated Ig-like receptor-1 Immune imbalance Child

分类号 R725.5 [医药卫生—儿科]

引文网络
相关文献

参考文献17

1MeMillan R. The role of antiplatelet autoantibody assays in the diagnosis of immune thrombocytopenic purpura[J]. Curr Hematol Rep, 2005, 4(2): 160-165.
2McKenzie CG, Guo L, Freedman J, et al. Cellular immune dysfunction in immune thrombocytopenia (ITP)[J]. Br J Haematol, 2013, 163(1): 10-23.
3Walk J, Westerlaken GH, van Uden NO, et al. Inhibitory receptor expression on neonatal immune cells[J]. Clin Exp Immunol, 2012, 169(2): 164-171.
4Jansen CA, Cruijsen CW, de Ruiter T, et al. Regulated expression of the inhibitory receptor LAIR-1 on human peripheral T cells during T cell activation and differentiation[J]. J Immunol, 2007, 37(4): 914-924.
5Olde Nordkamp M J, van Roon JA, Douwes M, et al. Enhanced secretion of leukocyte-associated immunoglobulin-like receptor 2 (LAIR-2) and soluble LAIR-1 in rheumatoid arthritis[J]. Arthritis Rheum, 2011, 63(12): 3749-3757.
6Zhang Y, Ding Y, Huang Y, et al. Expression of leukocyte- associated immunoglobulin-like receptor-1 (LAIR-l) onosteoclasts and its potential role in rheumatoid arthritis[J]. Clinics (Sao Paulo), 2013, 68(4): 475-481.
7Colombo BM, Canevali P, Magnani O, et al. Defective expression and function of the leukocyte associated Ig-like receptor 1 in B lymphocytes from systemic lupus erythematosus patients[J]. PLoS ONE, 2012, 7(2): e31903.
8Rodeghiero F, Stasi R, Gernsheimer T, et al. Standardization of terminology, definitions and outcome criteria in immune thrombocytopenic purpura of adults and children: report from an international working group[J]. Blood, 2009, 113(11): 2386-2393.
9刘晓帆,薛峰,张磊,周雪丽,刘永泽,刘葳,陈云飞,黄月婷,杨仁池.血小板膜糖蛋白特异性抗体在原发免疫性血小板减少症患者中的分布及与疗效的关系[J].中华血液学杂志,2013,34(7):610-613. 被引量：14
10周燕,钟周琳,申卫东.ITP患者血小板特异性自身抗体的检测及意义[J].广西医学,2010,32(6):708-709. 被引量：8

二级参考文献22

1张威,程大卫,周有宁,王兆钺,阮长耿.特发性血小板减少性紫癜自身抗体的特异性免疫诊断[J].苏州大学学报（医学版）,2004,24(5):678-680. 被引量：3
2涂亚兰.CD_4^+CD_(25)^+调节性T细胞在儿童特发性血小板减少性紫癜患者中的表达及意义[J].现代中西医结合杂志,2007,16(11):1454-1455. 被引量：4
3Mc Millan R,Wang L,Tani P.Prospective evaluation of the immunobead assay for the diagnosis of adult chronic immune thrombocytopenic purpura(ITP)[J].J Thromb Haemost,2003,1(3):485-491.
4Ruyi HE,Diane MR,Charles EJ,et al.Spectrum of Ig class,specificities,and titers of serum antiglycoroteins in chronic idiopathic thrombocytopenic purpura[J].Blood,1994,83(4):1 024-1 032.
5张之南.血液病诊断及疗效标准[M].3版.北京:科学出版社.2005:279-289.
6张之南,沈悌.血液病诊断及疗效标准[M].第3版.北京:科学出版社,2008.232-235.
7Cooper N, Bussel J. The pathogenesis of immune thrombocytopaenie purpura. Br J Haematol, 2006;133(4) :364-374.
8Zhou B, Zhao H, Yang RC, et al. Multi-dysfunctional pathophysiology in ITP. Crit Rev Oncol Hematol, 2005;54(2) :107-116.
9Chanock S. The etiology of childhood immune thrombocytopenic purpura: how complex is it? J Pediatr Hematol Oncol, 2003; 25 Suppl 1 :S7-10.
10Panitsas FP, Theodoropoulou M, Kouraklis A, et al. Adult chronic idiopathic thrombocytoponic purpura (ITP) is the manifestation of a type-1 polarized immune response. Blood, 2004; 103 (7): 2645-2647.