摘要
目的：研究端粒酶活性及 p53异常在非小细胞肺癌 (nonsmall cell lung cancer, NSCLC)中的表达及其相互关系。方法：用端粒重复扩增（ telomeric repeat amplification protocol, TRAP）法检测 NSCLC组织、癌旁非癌肺组织、肺良性病变组织端粒酶活性，用 Western blot印迹法检测 NSCLC组织 p53蛋白表达。结果：端粒酶活性表达率为： NSCLC 93.02％ (80/86)，癌旁非癌肺组织 9.33％ (7/75)，肺良性病变组织 27.27％ (3/11)， NSCLC组织端粒酶活性表达率显著高于癌旁非癌肺组织及肺良性病变，端粒酶活性表达率在不同组织类型、细胞分化、肿瘤大小、病理分期间差别不显著； NSCLC p53异常为 51.42％（ 36／ 70）， p53异常在Ⅰ、Ⅱ期与Ⅲ、Ⅳ期之间，Ⅰ级与Ⅱ、Ⅲ级之间差异有统计学意义；端粒酶活性表达与 p53异常无显著关联（ P >0.05）；结论： NSCLC标记物中，端粒酶活性表达较 p53敏感， p53对 NSCLC分期分级有帮助，端粒酶活性表达与 p53异常在 NSCLC发生发展中可能是两个独立的因素。
Objective： This study was designed to investigate telomerase activity and p53 abnormality in the patients with nonsmall cell lung cancer (NSCLC) and their association. Methods： Telomeric repeat amplification protocol (TRAP) assay was used to detect the telomerase activity in NSCLC tissues, tumor-adjacent non-cancer lung tissues, lung benign lesions; Western blot was used to examine p53 protein in NSCLC. Results： Telomerase activity expressions frequency were： 93.02％ (80/86) for NSCLC; 9.33％ (7/75) for tumor-adjacent non-cancer lung tissues;27.27％ (3/11) for lung benign lesions. Frequency of telomerase activity expression in NSCLC was much higher than that in tumor-adjacent non-cancer lung tissues or lung benign lesions, but was not significant different between histological types, differentiations, tumor size, and pathologic stages. The frequency of p53 abnormality in NSCLC was 51.42％（ 36／ 70） . The differences of p53 abnormality between pathologic StageⅠ Ⅱ andⅢ Ⅳ , GradeⅠ andⅡ Ⅲ have statistic significance. But there was no significant association between the telomerase activity expression and p53 protein abnormality. Conclusions： In the markers of NSCLC, telomerase activity expression is more sensitive than p53 abnormality, but p53 is more helpful in pathologic stages and grades. In genesis and proceeding of NSCLC, telomerase activating and p53 abnormality maybe two independent factors.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2001年第3期301-304,共4页
Chinese Journal of Cancer
