摘要
目的：研究胃、大肠癌与端粒酶表达活性及ＤＮＡ倍体的关系。方法：端粒重复扩增－酶联免疫吸附法（ＴＲＡＰ－ＥＬＩＳＡ法）检测肿瘤及无瘤残端粘膜端粒酶活性，流式细胞术检测肿瘤的ＤＮＡ倍体。结果：肿瘤瘤体端粒酶阳性者占８４．０％（４２／５０），无瘤残端酶阳性者占２．０％（１／５０）；端粒酶阳性肿瘤中异倍体肿瘤占５２．３８％（２２／４２），而端粒酶阴性肿瘤无异倍体出现；端粒酶阳性瘤体平均直径 ６．５ｃｍ，阴性瘤体平均直径 ３． ９ｃｍ；端粒酶阳性肿瘤淋巴转移率为 ５９.５％（２５／４２），阴性者转移率为 １２． ５％（１／８），四者差别均有显著意义， Ｐ值分别为： Ｐ＜０．００５，Ｐ＜０．０５，Ｐ＜０．００２，Ｐ＜０．０５。结论：胃癌及大肠癌端粒酶活性升高，酶阳性肿瘤异倍体发生率高，且瘤体大，淋巴转移率高，说明端粒酶的激活在恶性肿瘤的发生发展中是重要因素，并且与疾病的进程相关。
Objectives: To study the relation between telomerase activity or DNA ploridy and progression of gastric cancer and colorectal cancer. Methods: Telomerase activity was detected using telomeric repeat amplification protocal-enzyme linked immunosorbent assay (TRAP-ELISA ). The DNA content was detected using flow cytometry. Results: Most of the carcinomas (42/50) were telomerase positive, but the telomerase activity was observed infrequently in adjacent mucosa (1 /50) (P < 0.005). In this study, the aneuploid carcinomas were 52.38% (22/ 42) in telomerase-positive group, but there were no aneuploid carcinoma in telomerase-negative group, (P<0.05). The mean diameter of telomerase-positive carcinomas was 6.5 cm, but the diameter of telomerase-negative carcinomas was 3. 9 cm (P <0.002). The percentage of lymph node metastasis in telomerase-positive carcinomas (25/42) was higher than that in telomerase-negative carcinomas (1/8), P <0.05. Conclusions: Telomerase is important in the carcinogenesis of gastric cancer and colorectal cancer, and it is correlated with the progression of the diseases.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2000年第1期42-44,65,共4页
Chinese Journal of Cancer
基金
河北省卫生厅基金!(98002号)
关键词
胃肿瘤
大肠肿瘤
端粒酶
DNA倍体
Gastric cancer
Colorectal cancer
Telomerase
Polymerase chain reaction
Flow cytometry
