摘要
目的：探讨三氧化二砷（ arsenic trioxide,As2O3）和高三尖杉（ homoharringtonine,HHT）抑制神经母细胞瘤（ Neuroblastoma,NB）增殖的机制。方法：应用 MTT(Methy thiazolyl tetrazolium)法和细胞凋亡检测，观察 As2O3和 HHT对 NB细胞株增殖的影响及其作用机制，应用 RT-PCR(Reverse-transcriptase polymerase chain reaction)和免疫组化检测 As2O3和 HHT处理后 NB细胞株 N-myc mRNA和蛋白表达的变化。结果： As2O3和 HHT均能通过诱导 SJ-N-SH和 IMR-32细胞凋亡而起到抑制细胞增殖的作用； As2O3处理后 N-myc mRNA和蛋白均先有上调然后回落。 HHT处理后有 N-myc蛋白水平下降。结论： N-myc在 As2O3和 HHT诱导的细胞凋亡中起作用，但启动 N-myc的机制可能不同。
Objective： This study was designed to explore the mechanism of inhibition of proliferation induced by arsenic trioxide (As2O3) and homoharringtonine in neuroblastoma. Method： Methy thiazoloyl tetrazolium (MTT) methods and analysis of apoptosis were used to evaluate the effect of As2O3 and homoharringtonine on the proliferation of NB cell lines and their mechanisms. Reverse-transcriptase polymerase chain reaction(RT-PCR) and immunohistochemistry techniques were used in NB cell lines treated with As2O3 and HHT to determine N-myc mRNA and protein expression. Results： Both As2O3 and HHT can significantly inhibit the proliferation of neuroblastoma cell lines and induce apoptosis of SJ-N-SH and IMR-32 cells. In As2O3 treated cell line, at transcription and protein level, N-myc expression is up-regulated at first and then down-regulated to a lower level. HHT can down-regulate N-myc protein. Conclusion： N-myc may play some roles in apoptosis induced by As2O3 and HHT, but the mechanisms initiating N-myc gene may be different.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2001年第3期239-244,共6页
Chinese Journal of Cancer
基金
上海市百人计划项目(98-BR036)
