摘要
研究了长心卡帕藻（ Kappaphycus alvarezii）外植体切段的适宜生长温度以及诱导温度、切断长度、切段方式（与藻体纵轴呈90°横切、与藻体纵轴呈45°斜切）等因子对芽发生的影响。结果显示，在试验范围内，长心卡帕藻外植体切段的适宜生长温度为15～25℃，最适温度为20℃。诱导温度、切断长度、切段方式3种因子对外植体切段的出芽效果都存在显著影响，且各因子交互影响差异极其显著（P〈0．01），较高的培养温度对于出芽具有明显的促进作用，25℃下试验组个体平均出芽数均高于20℃（P〈0．01）；横切可促进外植体切段出芽，相同切段长度下，横切试验组个体平均出芽数均大于斜切试验组（P〈0．01）；短切段有助于提高出芽的数量，在相同切段方式下，1cm切段组个体平均出芽数显著优于3cm和5cm切段组（P〈0．01）；25℃、1cm、横切为长心卡帕藻最优出芽诱导条件，个体平均出芽数最高，达到平均1．50个。不同切段长度对芽的发生部位具有明显的影响，1cm和3cm切段组均为切面的髓部再生芽，而5cm切段组则包括切面的髓部再生芽和非切面的皮层新生芽。
We discussed the effects of induction temperature, cutting length and cutting way （ transected cutting and inclined cutting by 90 and 45 degree angles with algae body on the vertical axis, respectively） on growth and bud formation of Kappaphycus alvarezii explants. The results show that under the test conditions, the suitable growth temperature for K. alvarezii was 15 -25 ℃, the optimum temperature was 20 ℃. The effects of induction temperature, cutting length and cutting way for bud formation of K. alvarezii explants were significant, and interaction among each factor was very significantly different （P 〈 0. 01 ）. The cultivation at higher temperature obviously promoted the budding, and the average number of buddings at 25 ℃ was more than that at 20 ℃ （P 〈0. 01 ）. Transected cutting promoted the budding of explants, and by the same cutting length, the average number of buddings in crosscutting group was more than that in inclined cutting group （ P 〈 0.01 ）. Short cutting helped to increase the number of buddings by the same cutting way, 1 cm group obtained significantly more buddings than 3 cm and 5 cm groups （ P 〈 0. 01 ）. Besides, the individuals had the most aver- age number of buddings in 1 cm group at 25 ℃ （ average 1.50）. Different cutting lengths had obvious effect on the budding position. The explants in 1 cm and 3 cm groups formed regeneration buds on the section area, while those in 5 cm group formed regeneration buds on the section area and newborn buds on the cortex.
出处
《南方水产科学》
CAS
CSCD
北大核心
2014年第2期36-41,共6页
South China Fisheries Science
基金
海洋公益性行业科研专项经费项目(201405020)
国家大学生创新性实验计划项目(1111010507)
海南省科学事业费项目(11-20410-0015)
广东省海洋重大项目(2010B020201015)
中央高校基本科研业务费项目(201262003)
关键词
长心卡帕藻
外植体
组织培养
再生芽
新生芽
Kappaphycus alvarezii
explants
tissue culture
regeneration bud
newborn bud
