摘要
为进一步优化长心卡帕藻(Kappaphycus alvarezii)室内人工扩繁技术,通过开展控制试验,分别探究温度和光照对长心卡帕藻生长、色素和藻胆蛋白含量的影响。结果表明,温度为27℃时,长心卡帕藻相对生长率显著高于其他温度试验组(P<0.05),藻体的叶绿素a和类胡萝卜素含量也较高,说明温度适度升高会提升藻体色素含量的积累水平,但培养温度差异对藻胆蛋白含量的影响较小,各组藻胆蛋白含量差异不显著。在同一温度下,长心卡帕藻的相对生长率随光照强度变化呈先升后降的趋势,光照强度在4000~7000 lx时藻体能保持较快的生长;藻体叶绿素a含量在光照强度为1000 lx时显著高于其余光照组,说明适宜的低光照会促进长心卡帕藻合成更多的叶绿素a;光照强度为4000 lx时,藻体的藻胆蛋白含量明显高于其他光照组,光照强度的变化能引起长心卡帕藻的藻胆蛋白含量波动。
An attempt was made to further optimize the indoor artificial propagation technology of Kappaphycus alvarezii.The effects of temperature and light on the growth,pigment and phycobiliprotein content of K.alvarezii were determined by conducting control experiments.The results showed that when the temperature was 27℃,the relative growth rate was significantly higher than that of other temperature test groups(P<0.05),and that the contents of chlorophyll a and carotenoids in the algae were also higher,indicating that an appropriate increase of temperature would increase the accumulation level of pigment content in the algae,but the difference in culture temperature had little effect on the content of phycobiliprotein,and there was no significant difference in the content of phycobiliprotein in each group.At the same temperature,the relative growth rate of K.alvarezii increased first and then decreased with the change of light intensity.When the light intensity was 4000 lx to 7000 lx,the algae grew faster,and the content of chlorophyll a in the algae was significantly higher than that in the other light groups when the light was 1000 lx,indicating that the appropriate low light intensity would promote the synthesis of more chlorophyll a in K.alvarezii.When the light intensity was 4000 lx,the phycobiliprotein content of the algae was significantly higher than that of the other light groups,and the change of light intensity could cause the fluctuation of the phycobiliprotein content of K.alvarezii.
作者
吴翔宇
杨涵
黄良夫
唐贤明
杨守国
WU Xiangyu;YANG Han;HUANG Liangfu;TANG Xianming;YANG Shouguo(Hainan Academy of Ocean and Fisheries Sciences/Hainan Key Laboratory of Technology for Tropical Seawater Aquaculture,Haikou,Hainan 571126,China;College of Marine Biology and Fisheries,Hainan University,Haikou,Hainan 570228,China)
基金
海南省自然科学基金青年基金项目(322QN421)
2024年海南省科技条件平台专项(重点实验室)建设项目(ZDSYS202408)
现代农业产业技术体系专项资金项目(CARS-50)。
关键词
长心卡帕藻
温度
光照
生长
色素
Kappaphycus alvarezii
temperature
light intensity
growth
pigment
