摘要
为了研究饲粮添加L-精氨酸(Arg)或N-氨甲酰谷氨酸(NCG)对环江香猪肌肉脂肪酸含量和脂质代谢相关基因表达的影响,选用初始体重为(28.14±3.45)kg的环江香猪24头,随机分为3组,每组4个重复,每个重复2头猪。Arg组和NCG组分别在基础饲粮中添加0.83%L-Arg+0.88%米糠和0.08%NCG+1.71%L-丙氨酸,对照组在基础饲粮中添加1.71%L-丙氨酸。饲喂45 d后称取体重,放血处死,采集肝脏和背最长肌,测定背最长肌中脂肪酸的含量、肝脏和背最长肌中脂质代谢相关基因的表达。结果表明,与对照组相比,饲粮添加Arg可显著降低肌肉C18∶0含量(P<0.05),显著下调肝脏乙酰CoA羧化酶α(ACCα)以及肌肉ACCα和过氧化物酶增生物激活受体γ(PPARγ)mRNA表达(P<0.05),显著上调肝脏PPARγmRNA表达(P<0.05);饲粮添加NCG可显著下调肝脏ACCα和脂蛋白脂酶(LPL)以及肌肉ACCα、脂肪酸合成酶(FAS)和LPL mRNA表达(P<0.05),显著上调肌肉PPARγmRNA表达(P<0.05)。上述结果提示,饲粮添加Arg或NCG可调节肝脏和背最长肌中脂质代谢相关基因的表达,并降低背最长肌中C18∶0的含量。
This study was conducted to determine the effects of dietary supplemented with L-arginine （Arg） or N-carbamylglutamate （NCG） on intramuscular contents of fatty acids and the gene expression levels related to lipid metabolism in Huanjiang mini-pigs. Tweety-four Huanjiang mini-pigs with initial body weight （BW） of （28.14 ±3.45 ） kg were randomly assigned into 3 groups with 4 replicates in each group and 2 pigs per repli- cate, representing Arg, NCG and control groups, and pigs were supplemented with 0.83 % L-Arg ＋0. 88% rice bran, 0.08% NCG ±71% L-alanine, and 1.71% L-alanine in a basal diet, respectively. After a 45- day feeding period, the liver and longissimus dorsi were obtained for determining intramuscular contents of fat- ty acids and the gene expression levels related to lipid metabolism. The results showed that compared with the control group, dietary supplemented with Arg significantly decreased muscle content of C18：0 （P 〈 0.05 ）, as well as the expression levels of acetyl-CoA carboxylase α （ACCα） mRNA in liver, and ACCα and peroxisome proliferator activated receptor-γ （PPARγ） mRNA in muscle （P 〈0.05）, while significantly increased the ex- pression level of PPARy mRNA in liver （ P 〈 0.05） ; dietary supplementation of NCG significantly decreased the expression levels of ACCα and lipoprteinlipase （LPL） mRNA in liver （P 〈 0.05）, as well as ACCα, fatty acid synthetase （FAS） and LPL mRNA in muscle, while significantly increased the expression level of PPARy mRNA in muscle （ P 〈 0.05）. These results indicate that dietary supplemented with Arg or NCG have a regu- lating effect on gene expression related to lipid metabolism in the liver and longissimus dorsi, and decrease the content of C18：0 in longissimus dorsi.
出处
《动物营养学报》
CAS
CSCD
北大核心
2014年第4期1055-1060,共6页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家973计划课题(2012CB124704、2013CB127305)
国家自然科学基金项目(31270044、31101729)
