摘要
盐胁迫环境下植物活性氧的代谢及其清除能力是衡量植物抗性的主要指标之一,不同浓度盐胁迫对植物生理代谢的影响存在差异。以美蕊花同一无性系幼苗为材料,研究不同浓度NaCl(0、25、50、100、200、300和400 mmol·L-1)胁迫下,美蕊花幼苗叶片活性氧代谢及保护酶活性0-72 h变化规律,结果表明:随着盐胁迫浓度的增加和胁迫时间的延长,过氧化氢(H2O2)、丙二醛(MDA)含量和超氧阴离子(O-2)合成速率均显著上升;超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性呈先上升后下降的变化趋势,SOD、CAT和POD活性达到峰值的时间随盐胁迫浓度增加而提前。因此在胁迫中前期(0-48 h),中度(100、200 mmol·L-1)轻度(25、50 mmol·L-1)的盐胁迫下活性氧的清除系统是有效的。
The reactive oxygen metabolism along with its scavenging activity under salt stress is one of the main index to measure plant resistance and the effect of salt stress on plant physiological metabolism differs in salt concentration .In the study , Calliandra haematocephala seedlings were grown in sand to investigate the effects of reactive oxygen , lipid peroxidation and protective enzymes activities in leaves under salt stress for 72 h with seven concentrations：0, 25, 50, 100, 200, 300 and 400 mmol· L^-1 .The experi-mental results indicated that hydrogen peroxide （ H2 O2 ） and malondlaldehyde （ MDA） contents and superoxide dismutase （ O2-） composition rate in the leaves quantitatively increased with the concentration of salt over the time course .The activities of superoxide dismutase （ SOD） , catalase （ CAT） and peroxidase （ POD） in the leaves increased at the early stage and then decreased gradually . The occurrence of peak activities of SOD , CAT and POD in the leaves was earlier with increased salt stress .The protective enzymes in leaves of C.haematocephala are important for evaluating the defense response against oxidation damage induced by slight （25, 50 mmol· L^-1） and medium （100, 200 mmol· L^-1） salt stress at the early stage （0-48 h）.
出处
《福建林学院学报》
CSCD
北大核心
2014年第2期171-175,共5页
Journal of Fujian College of Forestry
基金
福建省科技重大专项(2006NZ0001)
关键词
美蕊花
盐胁迫
活性氧
保护酶
Calliandra haematocephala
salt stress
reactive oxygen species
protective enzymes
