摘要
目的 :建立三两半药酒中齐墩果酸含量测定方法。方法 :HPLC法 ,以Hypersil(4 .6× 2 5 0mm ,5 μm)为色谱柱 ,甲醇 水 (90∶10 )为流动相 ,检测波长为 2 2 0nm。结果 :齐墩果酸测定线性范围为 9.6～ 48.0 μg/ml(r=0 .9998) ,平均回收率为 98.5 %。 结论 :该法简便 ,可用于测定三两半药酒中齐墩果酸的含量。
OBJECTIVE:To develope a method for the determination of olesnolic acid in Sanliangban Yaojiu.METHOD:HPLC was employed.The column was Hyperisil ODS(4.6×250mm,5μm),the mobile phase was methanol water(90∶10),and the detection wavelength was 220nm.RESULTS:The linear range was from 9.6 to 48.0μg/ml(r=0.9998),and the average recovery was 98.5%.CONCLUSION:The method is simple for the determination of oleanolic acid in Sanliangban Yaojiu.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2001年第1期61-62,共2页
Chinese Journal of Modern Applied Pharmacy
