摘要
目的观察加味参附颗粒对慢性心力衰竭大鼠的血流动力学、心肌线粒体蛋白浓度及Na+-K+-ATP酶、Ca2+-ATP酶活性的影响。方法雄性SD大鼠50只,随机分为5组:正常对照组,模型组,曲美他嗪组及加味参附颗粒高、低剂量组,每组各10只。采用腹主动脉缩窄法建立心衰模型,预处理8周后,测定心衰大鼠心率、左室内最大上升和下降速率(±dp/dtmax)、血压、心肌线粒体蛋白浓度及Na+-K+-ATP酶、Ca2+-ATP酶活性。结果加味参附颗粒高、低剂量组心率、血压均低于模型组(P<0.05,P<0.01),在改善心率方面优于曲美他嗪组(P<0.05)。加味参附颗粒高、低剂量组±dp/dtmax、心肌线粒体蛋白浓度及Na+-K+-ATP酶、Ca2+-ATP酶活性均高于模型组(P<0.05);与曲美他嗪组比较,差异无统计学意义(P>0.05)。结论加味参附颗粒可能通过改善心肌能量代谢,进而改善心衰大鼠血流动力学,提高心肌线粒体蛋白浓度及Na+-K+-ATP酶、Ca2+-ATP酶活性。
Objective To observe the influence of modified Shenfu granules （MSG） on chronic heart failure rat hemodynamics, myocardial mitochondrial protein concentration and the activities of Na＋-K＋-ATPase, Ca2＋-ATPase. Methods Fifty male SD rats were evenly and randomly divided into normal control group, model control group, western medicine group（treated with trimetazidine）, and high-dose and low-dose MSG groups. Rat heart failure model was established by abdominal aortic coarctation method. After treatment for 8 weeks, heart rate, the maximum left ventricular pressure increase and decrease rate （＋dp/dtmax）, blood pressure, cardiac mitochondrial protein concentration, and the activities of Na＋-K＋-ATPase and Ca2＋-ATPase of heart failure rats were measured. Results Heart rate and blood pressure in high, and low-dose MSG groups were lower than those in the model group（P 〈 0.05, P 〈 0.01）, and MSG had better effect on improving heart rate than trimetazidine （P 〈 0.05）. High-, and low-dose MSG groups had better effect on increasing _＋ dp/dtn＋,）, cardiac mitochondrial protein concentration, and the activities of Na＋-K＋-ATPase and Ca2＋-ATPase than the model group （P 〈 0.05）, and the differences had no statistical significance when compared with trimetazidine group（P 〉 0.05）. Conclusion MSG ean improve the hemodynamics of heart failure rats, and increase myocardial mitochondrial protein concentration and the activities of Na＋-K＋-ATPase through the improvement of myocardial energy metabolism.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2014年第2期172-176,共5页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
广东科技计划课题(2010B030700021)
