摘要
目的 :对六味地黄汤活性部位 3A的免疫调节作用机理进行初步研究。方法 :观察 3A对环磷酰胺 (Cy)处理小鼠 ,荷瘤小鼠和快速老化小鼠的T细胞亚群 ,B细胞产生IgG水平 ,γ干扰素 (IFN γ)mR NA的表达水平的影响及体外活性研究。结果 :口服给予 3A对 3种模型小鼠脾脏T细胞亚群的变化均具有不同程度的恢复作用 ,并促进快速老化小鼠脾细胞分泌IgG ,提高Cy处理小鼠脾细胞IFN γmRNA的表达水平。结论 :3A对免疫功能的改善作用与调节T。
Objective: To investigate the immunomodulating mechanism of 3A, an active fraction extracted from the ethanol soluble portion of Liuwei Dihuang Decoction (LWDHD). Methods: Influence of 3A on T lymphocyte subsets, IgG production of B cells, interferon γ (IFN γ) mRNA expression in cyclophosphamide (Cy) treated mice, tumor bearing mice and senescence accelerated mouse prone/8 (SAMP8) were assessed in vivo and in vitro. Results: Oral administration of 3A could elevate the decreased T lymphocyte subsets in all model mice, it also could promote the IgG secretion of splenic cells in SAMP8 and increase IFN γ mRNA expression in Cy treated mice. Conclusion: The immunomodulating effect of 3A is related with its effects in regulating ratio of T and B cells or in improving their functions.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2001年第2期119-122,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家重点基础研究发展规划项目!(No .G1 9990 5440 1 )
"九五"国家攀登计划预选项目资助课题
关键词
六味地黄汤
活性部位
免疫调节
T细胞亚群
IGG
Γ干扰素
Liuwei Dihuang Decoction, active fraction, immuno modulating, T lymphocyte subsets, immunoglobulin G, interferon γ, RT polymerase chain reaction
