摘要
目的：研究中国南海简易扁板海绵Plakortis simplex的化学成分.方法：运用正相硅胶柱色谱、反相硅胶柱色谱（ODS）、凝胶柱色谱（Sephadex LH-20）以及高效液相色谱（HPLC）等多种手段对中国南海简易扁板海绵P.simplex的化学成分进行分离纯化;通过理化性质、波谱分析方法并结合文献对照,鉴定化合物的结构.结果：从该海绵的甲醇提取物中共分离鉴定了7个化合物：（22E）-5α,8α-epidioxyergosta-6,22-dien-3β-ol（1）,3-吲哚甲酸（2）,3-吲哚甲醛（3）,monotriajaponide A（4）,methyl（2Z,6R,8R,9E）-3,6-epoxy-4,6,8-triethyl-2,4,9 dodecatrienoate（5）,methyl（2Z,6R,8S）-3,6-epoxy-4,6,8-triethyldodeca-2,4-dienoate （6）,methyl（2Z,6R,8S）-4,6-diethyl-3,6-epoxy-8-methye（7）.结论：化合物1～7均为首次从该种海绵中分离得到.
Objective：To investigate the chemical constituents of the sponge Plakortis simplex from the south China sea.Method：Column chromatography on silica gel,reversed-phase silica gel （ODS）,Sephadex LH-20 and high performance liquid chromatography （HPLC） was used to study the chemical constituents of the sponge P.simplex collected from the south China sea.The structures of these compounds were elucidated on the basis of physicochemical and spectral analysis.Result：Seven compounds were isolated from the methanol extract of P.simplex,and their structures were identified as （22E）-5α,8α-epidioxyergosta-6,22-dien-3β-ol （1）,indole-3-carboxylic acid （2）,indole-3-carboxaldehyde （3）,monotriaj-aponide A （4）,methyl （2Z,6R,8R,9E）-3,6-epoxy-4,6,8-triethyl-2,4,9-dodec-atrienoate （5）,methyl （2Z,6R,8S）-3,6-epoxy-4,6,8-triethyldodeca-2,4-dienoate （6）,methyl （2Z,6R,8S）-4,6-diethyl-3,6-epoxy-8-methye （7）.Conclusion：7 compounds were isolated from P.simplex for the first time.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第8期99-102,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家"十二五"支撑计划项目(2012BAD38B09)
上海市科委工程中心建设项目(11DZ2280300)
关键词
海绵
化学成分
结构鉴定
sponge
chemical constituents
structural identification
