摘要
目的比较高效液相色谱(HPLC)和荧光偏振免疫(FPIA)测定血清万古霉素浓度方法的相关性。方法 30例患者的血清分别用HPLC和FPIA测定万古霉素浓度,对结果进行相关性分析。结果 HPLC测得万古霉素浓度为(30.04±14.02)μg/ml,FPIA测得值为(29.67±13.96)μg/ml,两种方法差值均值为(0.37±1.46)μg/ml,两种方法的线性相关系数r=0.9905。结论两种方法测定血清万古霉素药物浓度结果一致。
Objective The consistency of the results of serum vancomycin concentration the high-performance liquid chromatography（HPLC） and fluorescence polarization immunoassay（FPIA） were studied.Methods Serum vancomycin concentrations for 30 patients were measured by HPLC and FPIA. Correlation of results were analysised.Results Means for HPLC and standard deviation（SD） were（30.04±14.02） μg/ml. For FPIA,（29.67±13.96） μg/ml.The difference between the two methods＇ means were（0.37±1.46） μg/ml.The correlation coeffi cient of the two methods was 0.9905. Conclusion Measured serum vancomycin concentration results were consistent with each other.
出处
《空军医学杂志》
2013年第4期209-211,共3页
Medical Journal of Air Force
关键词
高效液相色谱法
荧光偏振免疫法
万古霉素
血药浓度
High per for mance liquid ch romatog raphy
Fluorescence polar ization immunoassay
Vancomycin
Serum drug concentration
