摘要
首次对肌红蛋白的同步荧光光谱进行了研究，并对肌红蛋白的荧光峰予以归属。当 为８０ ｎｍ时，３３５ｎｍ处的荧光峰来自肌红蛋白分子中色氨酸残基； 为２０ｎｍ时，３０８ｎｍ处的荧光峰主要为酪 氨酸残基的贡献，很小一部分是由色氨酸残基贡献的； 为４０ｎｍ时，分别在３２２和５９６ｎｍ处观察到两 个荧光峰，３２２ｎｍ的荧光峰为酪氨酸和色氨酸残基的共同贡献，５９６ｎｍ的荧光峰则归属为肌红蛋白分子 中血红素的贡献。
The synchronous fluorescence spectra of myoglobin were studies for the first time. The fluorescence peals observed in the spectra were assigned, When the wavelength interval (Delta lambda) is 80 nm, the main peak at 335 nm is originated from the tryptophan residues in the myoglobin molecule. When Delta lambda is 20 nn, the peak at 308 nm is mainly due to the tyrosine residues in the myoglobin molecule and in a small part due to the tryptophan residues. Two peaks at 322 and 596 nm were observed in the spectrum of myoglobin for Delta lambda = 40 nm. The peak at 322 nm is due to both tyrosine and tryptophan residues. The peak at 596 nm is attributed to the heme group in the myoglobin molecule.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2001年第2期219-221,共3页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金!(No.29973015)
江苏省教委自然科学基金
江苏省生物技术实验室资助课题。
