摘要
用ＭＡＬＤＩ－ＴＯＦ－ＭＳ测定了具有生物活性的重组人ＦＫ５０６（结合蛋白１２（ｒｈＦＫＢＰ１２）的分子量和胰 蛋白酶酶解的肽质量指纹谱，实验测定结果与理论计算值一致。证明其一级结构是正确的，在表达、复 性和纯化过程中没有氨基酸的丢失、变异和修饰。
The primary structure of rhFK506-Binding Protein (FKBP) 12 was studied with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS) by measuring its molecular weight and tryptic peptide mass fingerprinting.The calculated molecular weight of rhFKBP12 was 11819.5 and the measured value was 11818.0. The relative error was only 0.01%. The measured peptide mass fingerprinting of rhFKBP12 was identical with that deduced from its amino acid sequence.It proved that the primary structure of rhFKBP12 structure of rhFKBP12 was correct and there was no amino acid deletion,mutation and modification in its expression, refolding and purification.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2001年第2期125-127,共3页
Chinese Journal of Analytical Chemistry
