摘要
于1998年3月—1999年2月，在山东农业大学采集泰山赤鳞鱼，采用精子载体法进行MT-hGH基因导入的研究。将泰山赤鳞鱼的精子在保存液内与含有MT-hGH基因的重组质粒保温30min，然后用于人工授精，共孵化出363尾幼鱼。待鱼苗长至5cm左右时，对其中的39尾进行PCR和Southern杂交检测。结果表明，有7尾鱼苗带有MT-hGH基因，转基因鱼的阳性率为17.95%。
Transfering MT-hGH gene into Varicorhinus tracrolepis with sperms as vectors was carried out from March, 1998 to February, 1999. The Varicorhinus tracrolepis sperms were incubated with MT-hGH gene in dilute citrate solution for 30 minutes. Then they were used in artificial inse mination. 997 eggs were inseminated with the treated sperms and 755 of them were fertilized. 363 fish were hatched in 3—4 days. When the fish grew to about 5cm , genomic DNA was isolated from 39 fish. The samples were tested by PCR and Sout hern blot hybridization. The result shows that 7 fish contained MT-hGH gene. The positive rate was about 17.95%.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2001年第1期37-41,共5页
Oceanologia Et Limnologia Sinica
基金
山东省科学技术委员会攻关项目!D97060号。
