摘要
目的探讨左心疾病所致的肺动脉高压(PH-LHD)大鼠模型的制备方法。方法雄性未成年SD大鼠行冠状动脉开口以上的升主动脉缩窄手术来获得PH-LHD大鼠模型。模型组造模成功后分别在术后第1、3、5、8、15、22、36、50、64天进行体动脉压、右心室压监测。实验以第0天未经处理的大鼠及在第64天行假手术的大鼠作为对照。结果 SD大鼠行升主动脉缩窄手术后第64天,成功获得PH-LHD模型,表现为体动脉压力没有改变,右心室压力升高。结论冠状动脉以上的升主动脉的缩窄术是制作PH-LHD动物模型的理想方法。
Objective To discuss the method of establishing rats model of pulmonary hypertension associated with left heart diseases. Methods Juvenile SD rat model of PH - LHD was induced by supracoronary aortic banding. On dl, d3, dS, d8, d15, d22, d36, dS0, d64 after treatment, artery pressure and right ventricular pressure was chronologically collected. Rats without any treatment on dO and rats undergone sham operation on d64 were used as controls. Results On d64, unchanged artery pressure and increased right ventricular pressure were observed, indicating rat model of PH - LHD was successfully induced. Conclusion Supracoronary aortic banding is an ideal treatment to establish PH - LHD rat model.
出处
《临床和实验医学杂志》
2014年第5期352-355,共4页
Journal of Clinical and Experimental Medicine
基金
国家临床重点专科建设项目经费资助
福建省临床重点专科建设项目资助
关键词
大鼠
左心疾病所致的肺动脉高压
模型
Rats
Pulmonary hypertension associated with left heart diseases
Model
