摘要
目的 探讨Akt1基因敲除对坐骨神经结扎小鼠痛行为学的影响.方法 C57BL/6雄性小鼠随机分为Akt1基因敲除组（KO组,n=12）和野生组（WT组,n=12）.在小鼠右侧制作坐骨神经慢性挤压（chronic constriction injury,CCI）模型,测试术前1d和术后1d、3d、5d、7d、10d、14d、17d、21 d的机械缩足阈值（paw withdrawal mechanical threshold,PWMT）和热缩足潜伏期（paw withdrawal thermal latency,PWTL）.结果 KO组和WT组小鼠的两侧PWMT基础值[右侧：（0.89±0.15）g,（0.87±0.15）g；左侧：（0.97 ±0.19）g,（1.05±0,14）g,P＞0.05]和PWTL[右侧：（7.64±0.71）s,（7.56±0.68）s；左侧：（7.67±0.6）s,（7.64±0.64）s,P＞0.05]差异无统计学意义.术后各测试时间点KO组/WT组小鼠右侧的PWMT和PWTL与其基础值相比均明显减低（P＜0.05）,KO组左侧PWMT和PWTL与WT组小鼠相比差异无统计学意义（P＞0.05）,但是KO组右侧PWMT和PWTL较WT组明显降低（P＜0.05）.结论 Akt1基因敲除后会加重小鼠坐骨神经结扎所诱发的神经病理性疼痛.
Objective To investigate the effects of Aktl gene knockout on pain behavior induced by chro- nic constriction injury model of sciatic nerve（CCI）.Methods C57BL/6 male mice were randomly divided into Aktl knockout group （ KO group, n= 12） ,wiht type group（ WT group, n= 12） .All mice were made model of CCI in the right sciatic nerve. Each mouse received tests of the paw withdrawal mechanical threshold （PWMT）and the paw withdrawal thermal latency（PWTL） at the times of ld before and 1 d ,3 d,5 d,7 d, 10 d, 14 d, 17 d,21 d after sur- gery.Results For both KO group and WT group,the basic values of PMWT（right（0.89±0.15）g, （0.87±0.15）g; left （ 0.97±0.19 ） g, （1.05±0.14） g, P〉0.05 ） and PWTL（ right （ 7.64±0.71 ） s, （ 7.56±0.68 ） s ; left ： （ 7.67±0.6 ） s, （7.64±0.64） s, P〉0.05） showed no significantly statistical difference.Compared with WT group and the basic val- ue,PWMT and PWTL were significantly decreased after surgery in KO group （P〈0.05）.The PWMT and PWTI, of the left paw in KO group and WT group had no obvious statistieal difference （P〉0.05）.However, the PWMT and PWTL of the right paw significantly decreased in the two groups compared with left paw. Conclusion It aggra- vates the neuropathic pain induced by CCI in mice when the Aktl gene was knocked out.
出处
《中华行为医学与脑科学杂志》
CAS
CSCD
北大核心
2014年第3期236-238,共3页
Chinese Journal of Behavioral Medicine and Brain Science
基金
国家自然科学基金项目（81171047/H0903）
江苏省医学重点学科（XK201140）
江苏省医学重点人才（RC2011006）
