摘要
以毛花猕猴桃根为原料,乙醇为提取溶剂,超声辅助提取三萜类化合物,考察从毛花猕猴桃根中提取总三萜的最佳工艺。选取乙醇浓度、料液比、超声波功率和提取时间4个因素,通过单因素实验选取影响因素的水平,然后采用四因素三水平的响应面分析法(RSA)进行预测和分析,获得最佳优化条件为:乙醇浓度72%,料液比1∶20 g/mL,超声功率400 W,提取时间45 min,验证实验结果为12.65 mg/g,理论值与实际值接近。实验结果表明,根据Box-Benhnken中心组合设计试验结合响应面分析法可以较好的对毛花猕猴桃根中总三萜的提取工艺进行优化。
The objective of this study was to optimize the ultrasonic-assisted extraction of total triterpenoid from the root of Actinidia eriamha. Four factors, namely ethanol concentration, ratio of solvent to material, ultrasonic power and extraction time, were selected for the method optimization. Based on single factor experiments, the conditions of ultrasonic-assisted extraction for total triterpenoid from the root ofA. eriantha was optimized through response surface analysis （RSA） with four factors and three levels. The optimal extraction conditions were obtained as follows ：72% as ethanol concentration, 1：20 g/mL as material-liquid ratio, 400 W as ultrasonic power,45 min as extraction time. Under these conditions, the extraction yield of triterpenoid was determined to be 12.65 mg/g in the confirmatory experiment,which was close to the theoretical value. Hence, the ultrasonic extraction of triterpenoid extracted from the root of A. eriantha was successfully optimized by RSA.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2014年第3期431-437,共7页
Natural Product Research and Development
关键词
毛花猕猴桃
三萜类化合物
超声波提取工艺
响应面分析
Actinidia eriantha Benth.
triterpenoid
ultrasonic extraction
response surface analysis
