摘要
[目的 ]建立一种新的测试范围宽、分辨率高、重现性好的底液 ,采用电位溶出分析技术 ,检测人发锌含量。 [方法 ]应用微分电位溶出仪 (DPSA- 型 ) ,以磺基水杨酸 - KOH底液 ,选择试验条件 ,描制标准曲线 ,并进行样本前处理。 [结果 ]检出限为 0 .0 0 1μg/ m L ,线性范围 0 .0 2～ 0 .2μg/ m L ,回收率为 96 .2 %～ 10 6 .1% ,批内CV7.5 % ,30例人发样本测定结果与原子吸收法对照 ,两组之间无显著意义 (t<0 .0 5 ,P>0 .0 5 )。 15 1例健康学龄前儿童发锌测定结果符合我国儿童发锌偏低的报道。[结论 ]磺基水杨酸 - KOH底液在微分电位溶出法中 ,应用简便、快捷、重现性好 。
[Objective]To develop a new base solution with wide detection range,high resolution capacity and good repeatibility,for detecting zinc in human hair with potentiometric stripping analysis[Methods]The base solution of sulfosalicylic acid-KOH for differential potentiometric stripping analyzer (DPSA-Ⅱ)was applied in this assayThe screening of experimental conditions,preparing standard curve and pretreatment of samples were studied[Results]The detection limit,linear range,recovery rate and internal coefficient of variation was 0001 μg/mL,002~02 μg/mL,962%～1061% and 75% respectivelyThere was no significant differences between the levels of zinc in 30 human hair samples obtained from this assay and atomic absorption spectrophotometry (t<005,P>005)The levels of hair zinc of 151 healthy preschool children determined by this method accorded with the report that hair zinc of chinese children stayed in lower level[Conclusion]Application of base solution of sulfosalicylic acid-KOH in differential potentiometric stripping analysis, was easy and rapid to operate,present good repeatibility,and suitable for detection of human hair zinc levels among common laboratories
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2000年第6期364-366,共3页
Journal of Environment and Health
