摘要
采用COI基因序列和ISSR分子标记对分布于我国大连(DL)、乳山(RS)、盘锦(PJ)、青岛(QD)、盐城(YC)、晋江(JJ)和朝鲜西海岸(CX)7个地理种群双齿围沙蚕(Perinereis aibuhitensis)的遗传多样性和遗传结构进行了研究。10个ISSR引物在7个种群中共扩增出116条条带,其中多态性条带109条,总多态位点比率为93.97%,Nei's基因多样性指数为0.365 2,Shannon's信息指数为0.518 6,群体间遗传分化系数Gst值为0.246 0。数据表明24.60%的遗传分化发生在不同地理种群间,76.40%的遗传分化发生在整个种群内,群体内分化大于群体间分化。对7个群体70个个体COI基因序列进行分析,对比长度包括469个位点,其中变异位点68个,简约信息位点47个。在68个变异位点中,62个位点发生转换,6个位点发生颠换,简约信息位点率达9.611%,系统树分析表明供试群体内具有较高的遗传多样性,与ISSR分析结果相一致。该结果为科学有效的利用和保护沙蚕种质资源提供了理论依据。
ISSR molecular markers and CO I gene sequence were used to identify the genetic diversity and structure of 7 geographic populations in Perinereis aibuhitensis, which were sampled from Dalian （DL）, Rushan （RS）, Panjin （PJ）, Qingdao（ QD）, Yancheng（ YC）, Jinjiang（JJ） and west coast of North Korea（CX）. The ISSR results showed that there was high genetic diversity between the 7 geographic populations of P. aibuhitensis. 116 bands were amplified with 10 ISSR primers with 109 polymorphie bands. The total percentage of polymorphie loci was 93.97%. Nei＇s gene diversity was 0.365 2, Shannon＇s information index was 0.518 6. The coefficient of gene differentiation （Gst） was 0. 246 0. These data indicated that among-population component accounted for 24.60% of the total variation, while the within-population component accounted for 76.40%. The within-population genetic variation was larger than among-population. COI gene sequence was analyzed employing 70 individuals from 7 populations. There were 68 variable sites and 47 parsimony-information sites out of the total 469 sites. In 68 variable sites, there were 62 transition sites and 6 transversion sites, parsimony-information sites rate Pi was 9.611%. Phylogenetic tree analysis showed that there was high genetic diversity within the tested population, which was accord with the result of ISSR. This result has provided theoretical basis for effective utilization and protection of P. aibuhitensis resources.
出处
《中国农业科技导报》
CAS
CSCD
北大核心
2014年第1期139-147,共9页
Journal of Agricultural Science and Technology
基金
海洋公益性行业科研专项(201305002
201305043)
国家自然科学基金项目(41306138)资助
