摘要
在实验室条件下对 6 12株海洋细菌和 10株海水驯化淡水菌进行筛选 ,检测其快速降解水体中富营养有机物的能力。利用对虾饵料培养基、BOD仪、胞外酶检测等方法进行筛选 ,最后筛选到 10株对富营养有机物具有较高降解性能的细菌。所有的细菌均能产生明胶酶和脂酶 (Tween - 80 ) ,其中 9株细菌能产生淀粉酶 ,8株产生卵磷脂酶 ,2株产生酪蛋白酶 ,1株产生褐藻胶酶。通过测量BOD来衡量 10株细菌利用对虾饵料的效果 ,2天时能消化 46 .6 %～ 5 9.5 %的对虾饵料 ,5天时能消化 5 0 .8%～ 70 .2 %的对虾饵料。用常规生理生化方法将细菌鉴定到属 ,其中 3株为弧菌属细菌 (Vibriospp .) ,3株为假单胞菌属细菌 (Pseudomonasspp .) ,2株为发光杆菌属细菌 (Photobacteriumspp .) ,1株为气单胞菌属细菌 (Aeromonasspp .) ,1株为枯草杆菌 (Bacillussubtilis)。
bacterial strains from shrimp ponds and 10 freshwater strains which had been transplanted on the 2216E marine agar repeatedly were checked for their degrading abilities to the organic pollutants. 10 strains were selected by evaluating the growth on the shrimp feed medium, BOD in the liquid media and their extroenzymes activities. All these 10 strains were able to produce gelatinase, lipase (Tween-80) and 8 of these strains produced lecithinase, 9 produced amylase, 2 produced casinase, 1 produced alginase. All of them were able to degrade the feed of shrimp from 46.5% to 59.5% in two days and from 50.8% to 72.2% in five days. By the traditional physical and chemical methods, 3 of these strains were identified as Vibrio spp., 3 as Pseudomonas spp., 2 as Photobacterium spp., 1 as Aeromonas spp., and 1 as Bacillus subtilis.
出处
《水产学报》
CAS
CSCD
北大核心
2000年第4期334-338,共5页
Journal of Fisheries of China
基金
国家"九五"科技攻关资助!项目 (96 -0 0 5 -0 3 -0 1-0 3)
关键词
有机污染物降解细菌
饵料培养基
虾池
淤泥
organic pollutants degrading bacteria
shrimp feed medium
BOD
extra enzyme
bacterial identification
