摘要
比较了细胞松弛素B(CB)和 6 -二甲基氨基嘌呤 (6 -DMAP)通过抑制受精卵极体释放的方法批量诱导三倍体长牡蛎的养殖效果。长牡蛎卵子在 2 5℃的海水中受精 ,2 0～ 30min后 ,开始用浓度为 0 .5mg·L-1的CB处理 ,持续 18～ 2 2min ,受精卵处理密度为 4.0～ 4.5× 10 7个·L-1,三倍体产率为 6 5 .2 %～ 70 .1% ,面盘幼虫孵化率为 12 .3%～ 14.5 % ,诱导效率指数为 0 .0 9。 6 -DMAP的使用浓度为 40 0～ 42 0 μmol·L-1,受精卵处理密度为 3.0～ 3 .5× 10 7个·L-1,授精水温、处理起始和持续时间等与CB方法相同 ,三倍体产率为 5 8.7%～ 6 5 .4% ,面盘幼虫孵化率为 5 2 .1%～ 5 5 .4% ,诱导效率指数为 0 .32。两种方法的采苗率基本相同 ,采苗器为基质较硬的栉孔扇贝贝壳。海区养殖采用浮筏夹苗吊养技术 ,两种方法诱导的三倍体牡蛎养殖性状没有明显差别。通过比较CB和 6 -DMAP两种诱导方法及三倍体的养殖效果表明 ,后者具有更好的应用性。
Triploid of Pacific oyster Crassostrea gigas Thunberg was induced by blocking pb 2 with cytochalasin B (CB) and 6-dimethylaminopurine (6-DMAP) and the triploid seed had been farmed with suspended longline in the sea. The egg was fertilized at 25℃ and treated with 0.5mg·L -1 CB begun at 20-30min post fertilization lasted 18-22min. Incubated density of zygote when treatment was about 4.0-4.5×10 7ind·L -1 . Triploid yield was 65.2%-70.1% and velar larvae gain was 12.3%-14.5%, and the efficiency of triploid induction(ETI) was 0.09. The concentration of 6-DMAP used to the induction of triploid was 400-420 μmol·L -1 and the zygote density for treatment was 3.0-3.5×10 7ind·L -1 . Triploid yield was 58.7%-65.4%, velar larvae gain was 52.1%-55.4%, and ETI was 0.32. The surrounding temperature when fertilization, initial and lasting time for the triploid treatment were the same as those in CB. Spat harvest on the scallop shell which was as the collector was similar to both those in CB and 6-DMAP. The traits of the triploids induced by CB and 6-DMAP farmed for 14 months showed no significant difference in growth, meat harvest and condition index. Comprehensive comparison between the two methods showed that 6-DMAP was more applicable in triploid production than CB.
出处
《水产学报》
CAS
CSCD
北大核心
2000年第4期324-328,共5页
Journal of Fisheries of China
基金
国家"八六三"重大资助!项目 (86 3-819-0 1)
